Description
Monkeypox Virus (MPXV) M1R Protein IgG ELISA Kit
The Monkeypox Virus M1R Protein Human IgG ELISA Kit is a reliable and accurate kit designed for the detection of Monkeypox Virus M1R Protein-specific IgG antibodies in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with dependable and reproducible results for a variety of research applications.The Monkeypox Virus M1R Protein is a critical component of the Monkeypox Virus, a rare zoonotic disease with symptoms similar to smallpox. By detecting IgG antibodies specific to the M1R Protein, researchers can gain valuable insights into the immune response to Monkeypox Virus infection, aiding in the development of diagnostic tools and potential therapies.
With its advanced technology and easy-to-follow protocol, the Monkeypox Virus M1R Protein Human IgG ELISA Kit is a valuable tool for researchers studying Monkeypox Virus and related diseases, offering a comprehensive solution for the detection and quantification of Monkeypox Virus M1R Protein-specific IgG antibodies in human samples.
| Product Name: | Monkeypox Virus (MPXV) M1R Protein IgG ELISA Kit |
| Product Code: | MKRS0036 |
| Species Detected: | Human |
| Gene Symbols: | IGG |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
