Anti-MRPS27 Antibody (CAB11667)

Antibody Name:	Anti-MRPS27 Antibody
Antibody SKU:	CAB11667
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 255-414 of human MRPS27 (NP_055899.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, LO2, U-87MG, HeLa, A-431, HT-29, Mouse liver, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 255-414 of human MRPS27 (NP_055899.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YLDR ALQV MEKV AASP EDIK LCRE ALDV LGAV LKAL TSAD GASE EQSQ NDED NQGS EKLV EQLD IEET EQSK LPQY LERF KALH SKLQ ALGK IESE GLLS LTTQ LVKE KLST CEAE DIAT YEQN LQQW HLDL VQLI QREQ QQRE QAKQ EYQA QKAA KASA
Gene ID:	23107
Uniprot:	Q92552
Cellular Location:	Mitochondrion
Calculated MW:	47kDa/49kDa
Observed MW:	48kDa

Synonyms:

MRPS27, MRP-S27, S27mt

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	MRPS27: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.
UniProt Protein Details:	Protein type:Ribosomal; Mitochondrial; Translation Chromosomal Location of Human Ortholog: 5q13.2 Cellular Component: mitochondrial inner membrane; ribosome Molecular Function:protein binding Biological Process: mitochondrial translation; organelle organization and biogenesis
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2013]
UniProt Code:	Q92552
NCBI GenInfo Identifier:	209572725
NCBI Gene ID:	23107
NCBI Accession:	Q92552.3
UniProt Secondary Accession:	Q92552,Q6P1S1, B4DRT2,
UniProt Related Accession:	Q92552
Molecular Weight:	414
NCBI Full Name:	28S ribosomal protein S27, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein S27
NCBI Official Symbol:	MRPS27
NCBI Official Synonym Symbols:	S27mt; MRP-S27
NCBI Protein Information:	28S ribosomal protein S27, mitochondrial; mitochondrial 28S ribosomal protein S27
UniProt Protein Name:	28S ribosomal protein S27, mitochondrial
Protein Family:	28S ribosomal protein
UniProt Gene Name:	MRPS27
UniProt Entry Name:	RT27_HUMAN

	Western blot analysis of extracts of various cell lines, using MRPS27 antibody (CAB11667) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunofluorescence analysis of L929 cells using MRPS27 Rabbit pAb (CAB11667) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using MRPS27 Rabbit pAb (CAB11667) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-MRPS27 Antibody (CAB4527)

MRPS27 Antibody (PACO01108)

MRPS27 Antibody (PACO10611)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)