Anti-MRPS30 Antibody (CAB5841)

Antibody Name:	Anti-MRPS30 Antibody
Antibody SKU:	CAB5841
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 190-439 of human MRPS30 (NP_057724.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, HL-60, HeLa, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 190-439 of human MRPS30 (NP_057724.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PHNP ALAA AALD YRCP VHFY WVRG EEII PRGH RRGR IDDL RYQI DDKP NNQI RISK QLAE FVPL DYSV PIEI PTIK CKPD KLPL FKRQ YENH IFVG SKTA DPCC YGHT QFHL LPDK LRRE RLLR QNCA DQIE VVFR ANAI ASLF AWTG AQAM YQGF WSEA DVTR PFVS QAVI TDGK YFSF FCYQ LNTL ALTT QADQ NNPR KNIC WGTQ SKPL YETI EDND VKGF NDDV LLQI VHFL LNRP KEEK SQLL EN
Gene ID:	10884
Uniprot:	Q9NP92
Cellular Location:	Mitochondrion
Calculated MW:	50kDa
Observed MW:	54kDa

Synonyms:

MRPS30, MRP-S30, PAP, PDCD9, S30mt

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available.

UniProt Protein Function:	MRPS30: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 5q11 Cellular Component: mitochondrial inner membrane; ribosome Molecular Function:structural constituent of ribosome Biological Process: mitochondrial translation; apoptosis; organelle organization and biogenesis
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NP92
NCBI GenInfo Identifier:	116242771
NCBI Gene ID:	10884
NCBI Accession:	Q9NP92.2
UniProt Secondary Accession:	Q9NP92,Q96I91, Q96Q19, Q9H0P8, Q9NSF9, Q9NZ76,
UniProt Related Accession:	Q9NP92
Molecular Weight:	439
NCBI Full Name:	28S ribosomal protein S30, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein S30
NCBI Official Symbol:	MRPS30
NCBI Official Synonym Symbols:	PAP; PDCD9; S30mt; MRP-S30
NCBI Protein Information:	28S ribosomal protein S30, mitochondrial; programmed cell death 9
UniProt Protein Name:	28S ribosomal protein S30, mitochondrial
UniProt Synonym Protein Names:	Programmed cell death protein 9
Protein Family:	28S ribosomal protein
UniProt Gene Name:	MRPS30
UniProt Entry Name:	RT30_HUMAN

	Western blot analysis of extracts of various cell lines, using MRPS30 antibody (CAB5841) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using MRPS30 Antibody (CAB5841) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using MRPS30 Antibody (CAB5841) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using MRPS30 antibody (CAB5841). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug MRPS30 antibody (CAB5841). Western blot was performed from the immunoprecipitate using MRPS30 antibody (CAB5841) at a dilition of 1:1000.

Antibodies

MRPS30 Antibody (PACO02376)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)