The Mouse ICAM-1/CD54 (Intercellular Adhesion Molecule 1) ELISA Kit - High Sensitivity is a cutting-edge assay meticulously crafted for the precise assessment of Intercellular Adhesion Molecule 1 (ICAM-1 or CD54) levels in various mouse biological samples. ICAM-1 is a crucial adhesion molecule involved in immune responses, inflammation, and cellular interactions. By quantifying ICAM-1 levels with high sensitivity, researchers can elucidate its role in cell adhesion, immune cell recruitment, and inflammatory processes, providing valuable insights into disease progression and potential therapeutic interventions. This high-sensitivity ELISA kit empowers researchers to explore the intricate mechanisms underlying ICAM-1 signaling, paving the way for a deeper understanding of its impact on immune responses and inflammatory conditions. By accurately measuring ICAM-1 levels in mouse samples, researchers can unravel the complexities of immune cell interactions, vascular integrity, and inflammatory cascades, leading to novel discoveries in immunology and disease pathogenesis. The Mouse ICAM-1/CD54 ELISA Kit - High Sensitivity, developed by Assay Genie, ensures exceptional precision, sensitivity, and reproducibility, enabling reliable and high-quality results for advanced research applications. With stringent quality control standards and optimized performance, this kit provides robust and user-friendly protocols, making it an ideal choice for in-depth investigations into ICAM-1 biology and its implications in mouse models.
Product Name:
Mouse ICAM-1/CD54 ELISA Kit (High Sensitivity)
SKU:
AEES00607
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
