The Mouse IL-23 ELISA Kit - High Sensitivity is a specialized assay meticulously crafted for the precise and sensitive quantification of Interleukin-23 (IL-23) levels in a variety of mouse biological samples. IL-23, an essential cytokine associated with immune regulation and inflammation, plays a pivotal role in stimulating and maintaining pro-inflammatory responses, particularly the differentiation and function of Th17 cells. This crucial cytokine is involved in various immune-related processes, including host defense, autoimmunity, and inflammatory diseases. With its high sensitivity and accuracy, the Mouse IL-23 ELISA Kit - High Sensitivity provides researchers with a powerful tool to delve into the intricate mechanisms of immune modulation and inflammatory pathways mediated by IL-23. Accurate measurement of IL-23 levels using this kit is indispensable for unraveling the complexities of immune responses, understanding disease pathogenesis, and exploring potential therapeutic strategies for immune-related disorders and inflammatory conditions. Constructed to deliver exceptional specificity and reproducibility, this ELISA kit from Assay Genie ensures reliable and consistent results, enabling researchers to uncover the role of IL-23 in immune regulation and inflammatory processes with confidence. Manufactured under strict quality control measures and designed for user-friendly operation, this high-performance kit offers researchers a valuable resource for advancing immunological research and drug development initiatives.
Product Name:
Mouse IL-23 ELISA Kit (High Sensitivity)
SKU:
AEES00624
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
