The Mouse MPIF2 (Myeloid Progenitor Inhibitory Factor 2) ELISA Kit - High Sensitivity is a sophisticated research tool designed for the precise quantitative analysis of Myeloid Progenitor Inhibitory Factor 2 (MPIF2) levels in various biological samples derived from mouse models. MPIF2, a key factor involved in myeloid progenitor cell regulation, exerts inhibitory effects on myeloid cell development and differentiation, thereby influencing hematopoiesis and immune responses in mice. With a high sensitivity ELISA kit specifically tailored for MPIF2 detection, researchers can delve into the complex mechanisms governing myeloid progenitor cell regulation and hematopoietic processes in mouse experimental systems. By accurately measuring MPIF2 levels, researchers can elucidate its role in modulating myeloid cell function and immune responses, providing valuable insights into hematopoiesis and immune regulation dynamics in mouse models. The Mouse MPIF2 ELISA Kit - High Sensitivity by Assay Genie offers exceptional precision and accuracy, ensuring the generation of reliable and reproducible data. Manufactured to stringent quality control standards, this kit guarantees robust performance and ease of use, making it an ideal choice for researchers exploring myeloid cell biology, hematopoiesis, and immune regulation in mouse studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
