Anti-MSI1 Antibody (CAB2609)

Antibody Name:	Anti-MSI1 Antibody
Antibody SKU:	CAB2609
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MSI1 (NP_002433.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, 293T, Mouse brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MSI1 (NP_002433.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	METD APQP GLAS PDSP HDPC KMFI GGLS WQTT QEGL REYF GQFG EVKE CLVM RDPL TKRS RGFG FVTF MDQA GVDK VLAQ SRHE LDSK TIDP KVAF PRRA
Gene ID:	4440
Uniprot:	O43347
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	39kDa
Observed MW:	42KDa

Synonyms:

MSI1

Background:

This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.

UniProt Protein Function:	Musashi-1: RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'- GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'- [GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system. Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. Belongs to the Musashi family.
UniProt Protein Details:	Protein type:RNA-binding Chromosomal Location of Human Ortholog: 12q24 Molecular Function:RNA binding Biological Process: nervous system development
NCBI Summary:	This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq, Jul 2008]
UniProt Code:	O43347
NCBI GenInfo Identifier:	51316193
NCBI Gene ID:	4440
NCBI Accession:	O43347.1
UniProt Secondary Accession:	O43347,Q96PU0, Q96PU1, Q96PU2, Q96PU3,
UniProt Related Accession:	O43347
Molecular Weight:	39,125 Da
NCBI Full Name:	RNA-binding protein Musashi homolog 1
NCBI Synonym Full Names:	musashi RNA binding protein 1
NCBI Official Symbol:	MSI1
NCBI Protein Information:	RNA-binding protein Musashi homolog 1
UniProt Protein Name:	RNA-binding protein Musashi homolog 1
Protein Family:	RNA-binding protein
UniProt Gene Name:	MSI1
UniProt Entry Name:	MSI1H_HUMAN

	Western blot analysis of extracts of various cell lines, using MSI1 Polyclonal Antibody (CAB2609) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat lung using MSI1 antibody (CAB2609) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of U2OS cells using MSI1 antibody (CAB2609) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)