Anti-MSRB1 Antibody (CAB6737)

Antibody Name:	Anti-MSRB1 Antibody
Antibody SKU:	CAB6737
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-94 of human MSRB1 (NP_057416.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, SW620, Mouse brain, Mouse kidney, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-94 of human MSRB1 (NP_057416.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSFC SFFG GEVF QNHF EPGV YVCA KCGY ELFS SRSK YAHS SPWP AFTE TIHA DSVA KRPE HNRS EALK VSCG KCGN GLGH EFLN DGPK PGQS RF
Gene ID:	51734
Uniprot:	Q9NZV6
Cellular Location:	Cytoplasm, Nucleus, cytoskeleton
Calculated MW:	12kDa
Observed MW:	13kDa

Synonyms:

MSRB1, HSPC270, SELR, SELX, SEPX1, SepR

Background:

This gene encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein belongs to the methionine sulfoxide reductase (Msr) protein family which includes repair enzymes that reduce oxidized methionine residues in proteins. The protein encoded by this gene is expressed in a variety of adult and fetal tissues and localizes to the cell nucleus and cytosol.

UniProt Protein Function:	SEPX1: a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein belongs to the methionine sulfoxide reductase (Msr) protein family which includes repair enzymes that reduce oxidized methionine residues in proteins. The protein encoded by this gene is expressed in a variety of adult and fetal tissues and localizes to the cell nucleus and cytosol. [provided by RefSeq, Mar 2012]
UniProt Protein Details:	Protein type:Oxidoreductase; EC 1.8.4.- Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: cytoplasm; nucleus; actin cytoskeleton Molecular Function:zinc ion binding; peptide-methionine (R)-S-oxide reductase activity; actin binding Biological Process: protein repair; actin filament polymerization; innate immune response; response to oxidative stress
NCBI Summary:	The protein encoded by this gene belongs to the methionine-R-sulfoxide reductase B (MsrB) family. Members of this family function as repair enzymes that protect proteins from oxidative stress by catalyzing the reduction of methionine-R-sulfoxides to methionines. This protein is highly expressed in liver and kidney, and is localized to the nucleus and cytosol. It is the only member of the MsrB family that is a selenoprotein, containing a selenocysteine (Sec) residue at its active site. It also has the highest methionine-R-sulfoxide reductase activity compared to other members containing cysteine in place of Sec. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A pseudogene of this locus has been identified on chromosome 19. [provided by RefSeq, Aug 2017]
UniProt Code:	Q9NZV6
NCBI GenInfo Identifier:	182676387
NCBI Gene ID:	51734
NCBI Accession:	Q9NZV6.3
UniProt Related Accession:	Q9NZV6
Molecular Weight:	13kDa
NCBI Full Name:	Methionine-R-sulfoxide reductase B1
NCBI Synonym Full Names:	methionine sulfoxide reductase B1
NCBI Official Symbol:	MSRB1
NCBI Official Synonym Symbols:	SELR; SELX; SepR; SEPX1; HSPC270
NCBI Protein Information:	methionine-R-sulfoxide reductase B1
UniProt Protein Name:	Methionine-R-sulfoxide reductase B1
UniProt Synonym Protein Names:	Selenoprotein X; SelX
Protein Family:	Methionine-R-sulfoxide reductase
UniProt Gene Name:	MSRB1
UniProt Entry Name:	MSRB1_HUMAN

	Western blot analysis of extracts of various cell lines, using MSRB1 Antibody (CAB6737) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using MSRB1 antibody (CAB6737). Blue: DAPI for nuclear staining.

ELISA Kits

HumanÂ MSRB1Â (Methionine-R-sulfoxideÂ reductaseÂ B1)Â ELISAÂ KIT (HUFI04903)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)