Description
Mycobacterium tuberculosis esat6 Recombinant Protein (C-His)
Mycobacterium tuberculosis esat6 Recombinant Protein (C-His) is a recombinant protein expressed in E. coli, spanning Met1-Ala95, with a C-terminal His tag. Predicted molecular weight: 10.73 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. 6 kDa early secretory antigenic target (esat6) is a ~9 kDa protein. A secreted protein that plays a number of roles in modulating the host's immune response to infection as well as being responsible for bacterial escape into the host cytoplasm. For research use only.
Acts as a strong host (human) T-cell antigen. Inhibits IL-12 p40 (IL12B) and TNF expression by infected host (mouse) macrophages, reduces the nitric oxide response by about 75%. In mice previously exposed to the bacterium, elicits high level of IFN-gamma production by T-cells upon subsequent challenge by M.tuberculosis, in the first phase of a protective immune response. Higher levels (1.6-3.3 uM) of recombinant protein inhibit IFN-gamma production by host (human) T-cells and also IL-17 and TNF production but not IL-2; decreases expression of host ATF-2 and JUN transcription factors by affecting T-cell receptors signaling downstream of ZAP70, without cytotoxicity or apoptosis.
| Product Name: | Mycobacterium tuberculosis esat6 Recombinant Protein (C-His) |
| SKU: | GXRP08045 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Mycobacterium tuberculosis |
| Synonyms: | 6 kDa early secretory antigenic target, ESAT-6, esxA, esaT6, Rv3875, MTV027.10 |
| Tag: | C-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Ala95 |
| Calculated MW: | 10.73 kDa |
| UniProt: | P9WNK7 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
