null

Anti-MYL2 Antibody (CAB14188)

SKU:
CAB14188
Product Type:
Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 4 Anti-MYL2 Antibody CAB14188
  • Cell Biology Antibodies 4 Anti-MYL2 Antibody CAB14188
  • Cell Biology Antibodies 4 Anti-MYL2 Antibody CAB14188
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-MYL2 Antibody
Antibody SKU:CAB14188
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human MYL2
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Mouse, Rat
Positive Samples:Mouse heart, Mouse skeletal muscle, Rat heart, Rat skeletal muscle
Immunogen:Recombinant protein of human MYL2
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4633
Uniprot:P10916
Cellular Location:A band, Cytoplasm, myofibril, sarcomere
Calculated MW:18kDa
Observed MW:19kDa
Synonyms:MYL2, CMH10, MLC-2s/v, MLC2
Background:Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
UniProt Protein Function:MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
UniProt Protein Details:

Protein type:Contractile

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: sarcomere; cytoskeleton; myofibril; cytosol; myosin complex; A band; actin cytoskeleton

Molecular Function:actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding

Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; muscle filament sliding; cardiac muscle contraction; post-embryonic development

Disease: Cardiomyopathy, Familial Hypertrophic, 10

NCBI Summary:Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
UniProt Code:P10916
NCBI GenInfo Identifier:6166556
NCBI Gene ID:4633
NCBI Accession:P10916.3
UniProt Secondary Accession:P10916,Q16123,
UniProt Related Accession:P10916
Molecular Weight:166
NCBI Full Name:Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
NCBI Synonym Full Names:myosin, light chain 2, regulatory, cardiac, slow
NCBI Official Symbol:MYL2  
NCBI Official Synonym Symbols:MLC2; CMH10  
NCBI Protein Information:myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; RLC of myosin; myosin light chain 2; regulatory light chain of myosin; cardiac ventricular myosin light chain 2; slow cardiac myosin regulatory light chain 2; myosin, light polypeptide 2, regulatory, cardiac, slow
UniProt Protein Name:Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Protein Family:Myosin regulatory
UniProt Gene Name:MYL2  
UniProt Entry Name:MLRV_HUMAN
Anti-MYL2 Antibody (CAB14188)Western blot analysis of extracts of various cell lines, using MYL2 antibody (CAB14188) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-MYL2 Antibody (CAB14188)Immunofluorescence analysis of rat heart using MYL2 Rabbit pAb (CAB14188) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-MYL2 Antibody (CAB14188)Immunofluorescence analysis of mouse heart using MYL2 Rabbit pAb (CAB14188) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Related Products