null

Anti-ND5 Antibody (CAB8135)

SKU:
CAB8135
Product Type:
Antibody
Applications:
WB
IHC
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 11 Anti-ND5 Antibody CAB8135
  • Cell Biology Antibodies 11 Anti-ND5 Antibody CAB8135
  • Cell Biology Antibodies 11 Anti-ND5 Antibody CAB8135
  • Cell Biology Antibodies 11 Anti-ND5 Antibody CAB8135
  • Cell Biology Antibodies 11 Anti-ND5 Antibody CAB8135
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:MT-ND5 Rabbit Polyclonal Antibody
Antibody SKU:CAB8135
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 500-600 of mouse MT-ND5 (NP_904338.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse heart, Mouse brain, Rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 500-600 of mouse MT-ND5 (NP_904338.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IALE LNNL TMKL SMNK ANPY SSFS TLLG FFPS IIHR ITPM KSLN LSLK TSLT LLDL IWLE KTIP KSTS TLHT NMTT LTTN QKGL IKLY FMSF LINI ILII I
Gene ID:17721
Uniprot:P03915
Cellular Location:Mitochondrion inner membrane, Multi-pass membrane protein
Calculated MW:68kDa
Observed MW:67kDa
Synonyms:MT-ND5, MTND5, ND5
Background:
UniProt Protein Function:MT-ND5: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in MT-ND5 are a cause of Leber hereditary optic neuropathy (LHON). LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes. Defects in MT-ND5 are a cause of Leigh syndrome (LS). LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions. Defects in MT-ND5 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Defects in MT-ND5 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS). MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness. Belongs to the complex I subunit 5 family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase; EC 1.6.5.3; Mitochondrial

Chromosomal Location of Human Ortholog: -

Disease: Leber Optic Atrophy; Mitochondrial Myopathy, Encephalopathy, Lactic Acidosis, And Stroke-like Episodes

UniProt Code:P03915
NCBI GenInfo Identifier:6648059
NCBI Gene ID:4540
NCBI Accession:P03915.2
UniProt Secondary Accession:P03915,Q34773, Q8WCY3,
Molecular Weight:67,027 Da
NCBI Full Name:NADH-ubiquinone oxidoreductase chain 5
NCBI Synonym Full Names:mitochondrially encoded NADH dehydrogenase 5
NCBI Official Symbol:MT-ND5  
NCBI Official Synonym Symbols:MTND5; ND5  
NCBI Protein Information:NADH dehydrogenase, subunit 5 (complex I)
UniProt Protein Name:NADH-ubiquinone oxidoreductase chain 5
UniProt Synonym Protein Names:NADH dehydrogenase subunit 5
UniProt Gene Name:MT-ND5  
UniProt Entry Name:NU5M_HUMAN
Anti-ND5 Polyclonal Antibody (CAB8135)Western blot analysis of extracts of various cell lines, using MT-ND5 antibody (CAB8135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-ND5 Polyclonal Antibody (CAB8135)Immunohistochemistry of paraffin-embedded rat brain using MT-ND5 antibody (CAB8135) at dilution of 1:100 (40x lens).
Anti-ND5 Polyclonal Antibody (CAB8135)Immunohistochemistry of paraffin-embedded rat kidney using MT-ND5 antibody (CAB8135) at dilution of 1:100 (40x lens).
Anti-ND5 Polyclonal Antibody (CAB8135)Immunohistochemistry of paraffin-embedded human colon carcinoma using MT-ND5 antibody (CAB8135) at dilution of 1:100 (40x lens).
Anti-ND5 Polyclonal Antibody (CAB8135)Immunohistochemistry of paraffin-embedded human prostate cancer using MT-ND5 antibody (CAB8135) at dilution of 1:100 (40x lens).

Related Products