null

Anti-NDEL1 Antibody (CAB5776)

SKU:
CAB5776
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology
  • Developmental Biology Anti-NDEL1 Antibody CAB5776
  • Developmental Biology Anti-NDEL1 Antibody CAB5776
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-NDEL1 Antibody
Antibody SKU:CAB5776
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-345 of human NDEL1 (NP_110435.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:U-87MG, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-345 of human NDEL1 (NP_110435.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDGE DIPD FSSL KEET AYWK ELSL KYKQ SFQE ARDE LVEF QEGS RELE AELE AQLV QAEQ RNRD LQAD NQRL KYEV EALK EKLE HQYA QSYK QVSV LEDD LSQT RAIK EQLH KYVR ELEQ ANDD LERA KRAT IVSL EDFE QRLN QAIE RNAF LESE LDEK ESLL VSVQ RLKD EARD LRQE LAVR ERQQ EVTR KSAP SSPT LDCE KMDS AVQA SLSL PATP VGKG TENT FPSP KAIP NGFG TSPL TPSA RISA LNIV GDLL RKVG ALES KLAA CRNF AKDQ ASRK SYIS GNVN CGVL NGNG TKFS RSGH TSFF DKGA VNGF DPAP PPPG LGSS RPSS APGM LPLS V
Gene ID:81565
Uniprot:Q9GZM8
Cellular Location:Chromosome, Cytoplasm, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle
Calculated MW:37kDa/38kDa/40kDa
Observed MW:39kDa
Synonyms:NDEL1, EOPA, MITAP1, NDE1L1, NDE2, NUDEL
Background:This gene encodes a coiled-coil protein that plays a role in multiple processes including cytoskeletal organization, cell signaling and neuron migration, outgrowth and maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome X.
UniProt Protein Function:NDEL1: Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Belongs to the nudE family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Protease; Microtubule-binding

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: axon hillock; kinetochore; microtubule; kinesin complex; centrosome; neurofilament cytoskeleton; cytoplasm; leading edge; nucleolus; nuclear envelope; spindle; cytosol

Molecular Function:protein binding; microtubule binding; beta-tubulin binding; alpha-tubulin binding

Biological Process: cell migration; positive regulation of axon regeneration; central nervous system neuron axonogenesis; positive regulation of axon extension; establishment of chromosome localization; neuron migration; nuclear envelope disassembly; proteolysis; microtubule nucleation; inner cell mass cell proliferation; retrograde axon cargo transport; chromosome segregation; mitotic centrosome separation; establishment of mitotic spindle orientation; centrosome localization; neurofilament cytoskeleton organization and biogenesis; vesicle transport along microtubule; cerebral cortex radially oriented cell migration; mitotic cell cycle

NCBI Summary:This gene encodes a coiled-coil protein that plays a role in multiple processes including cytoskeletal organization, cell signaling and neuron migration, outgrowth and maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Mar 2012]
UniProt Code:Q9GZM8
NCBI GenInfo Identifier:74725006
NCBI Gene ID:81565
NCBI Accession:Q9GZM8.1
UniProt Secondary Accession:Q9GZM8,Q86T80, Q8TAR7, Q9UH50, B3KP93, D3DTS0, J3QT32
UniProt Related Accession:Q9GZM8
Molecular Weight:345
NCBI Full Name:Nuclear distribution protein nudE-like 1
NCBI Synonym Full Names:nudE neurodevelopment protein 1-like 1
NCBI Official Symbol:NDEL1  
NCBI Official Synonym Symbols:EOPA; NDE2; NUDEL; MITAP1; NDE1L1  
NCBI Protein Information:nuclear distribution protein nudE-like 1; protein Nudel; endooligopeptidase A; mitosin-associated protein 1; mitosin-associated protein MITAP1; nudE nuclear distribution E homolog-like 1; nudE nuclear distribution gene E homolog-like 1
UniProt Protein Name:Nuclear distribution protein nudE-like 1
UniProt Synonym Protein Names:Mitosin-associated protein 1
UniProt Gene Name:NDEL1  
UniProt Entry Name:NDEL1_HUMAN
Anti-NDEL1 Antibody (CAB5776)Western blot analysis of extracts of various cell lines, using NDEL1 antibody (CAB5776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-NDEL1 Antibody (CAB5776)Immunofluorescence analysis of U2OS cells using NDEL1 antibody (CAB5776). Blue: DAPI for nuclear staining.

Related Products