NDN Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, IHC, IF, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ug format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Product Name:
NDN Antibody (PACO58440)
SKU:
PACO58440
Size:
50μg
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant Human Necdin protein (1-98AA)
Immunogen Species:
Homo sapiens (Human)
Uniprot No:
Q99608
Form:
Liquid
Tested Applications:
ELISAIHCIF
Recommended Dilution:
IHC 1:200-1:500, IF 1:50-1:200
Synonyms:
HsT16328 antibody, NDN antibody, NECD_HUMAN antibody, Necdin antibody, Necdin homolog antibody, Necdin MAGE family member antibody, necdin related protein antibody, PWCR antibody
IHC image of PACO58440 diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO58440 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
