Anti-NDUFA8 Antibody (CAB12118)

Antibody Name:	Anti-NDUFA8 Antibody
Antibody SKU:	CAB12118
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 33-172 of human NDUFA8 (NP_055037.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, LO2, MCF7, Mouse heart, Mouse brain, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 33-172 of human NDUFA8 (NP_055037.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GAQC DKPN KEFM LCRW EEKD PRRC LEEG KLVN KCAL DFFR QIKR HCAE PFTE YWTC IDYT GQQL FRHC RKQQ AKFD ECVL DKLG WVRP DLGE LSKV TKVK TDRP LPEN PYHS RPRP DPSP EIEG DLQP ATHG SRFY FWTK
Gene ID:	4702
Uniprot:	P51970
Cellular Location:	Mitochondrion inner membrane, Mitochondrion intermembrane space, Peripheral membrane protein
Calculated MW:	20kDa
Observed MW:	19kDa

Synonyms:

NDUFA8, CI-19KD, CI-PGIV, PGIV

Background:

The protein encoded by this gene belongs to the complex I 19 kDa subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	NDUFA8: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFA8 subunit family.
UniProt Protein Details:	Protein type:Energy Metabolism - oxidative phosphorylation; Mitochondrial; Oxidoreductase; EC 1.6.5.3; EC 1.6.99.3 Chromosomal Location of Human Ortholog: 9q33.2 Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial respiratory chain complex I Molecular Function:NADH dehydrogenase (ubiquinone) activity; protein complex binding Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone
NCBI Summary:	The protein encoded by this gene belongs to the complex I 19 kDA subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq, Jul 2008]
UniProt Code:	P51970
NCBI GenInfo Identifier:	8039804
NCBI Gene ID:	4702
NCBI Accession:	P51970.3
UniProt Secondary Accession:	P51970,Q9Y6N0, B1AM93,
UniProt Related Accession:	P51970
Molecular Weight:	172
NCBI Full Name:	NADH dehydrogenase
NCBI Synonym Full Names:	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa
NCBI Official Symbol:	NDUFA8
NCBI Official Synonym Symbols:	PGIV; CI-19KD; CI-PGIV
NCBI Protein Information:	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; complex I-19kD; complex I-PGIV; complex I PGIV subunit; NADH:ubiquinone oxidoreductase PGIV subunit; NADH-ubiquinone oxidoreductase 19 kDa subunit
UniProt Protein Name:	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
UniProt Synonym Protein Names:	Complex I-19kD; CI-19kD; Complex I-PGIV; CI-PGIV; NADH-ubiquinone oxidoreductase 19 kDa subunit
Protein Family:	NADH dehydrogenase
UniProt Gene Name:	NDUFA8
UniProt Entry Name:	NDUA8_HUMAN

	Western blot analysis of extracts of various cell lines, using NDUFA8 antibody (CAB12118) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of H9C2 cells using NDUFA8 Rabbit pAb (CAB12118) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using NDUFA8 Rabbit pAb (CAB12118) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug NDUFA8 antibody (CAB12118). Western blot was performed from the immunoprecipitate using NDUFA8 antibody (CAB12118) at a dilition of 1:1000.

Antibodies

Anti-NDUFA8 Antibody (CAB1272)

NDUFA8 Antibody (PACO01146)

NDUFA8 Antibody (PACO10780)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)