Western blot analysis of extracts of various cell lines, using NDUFAB1 antibody (CAB22019) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The NDUFAB1 Polyclonal Antibody (CAB22019) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity when used in Western blot applications with human samples. By binding to NDUFAF1, it enables the detection and analysis of this protein in various cell types, making it ideal for studies in mitochondrial biology and metabolic disorders.NDUFAF1 is essential for the assembly and function of complex I in the electron transport chain, playing a key role in generating cellular energy through oxidative phosphorylation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NDUFAB1 Polyclonal Antibody
SKU:
CAB22019
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Predicted to enable acyl binding activity; acyl carrier activity; and fatty acid binding activity. Involved in mitochondrial respiratory chain complex I assembly and protein lipoylation. Located in mitochondrion and nucleoplasm. Part of mitochondrial respiratory chain complex I. Colocalizes with mitochondrial large ribosomal subunit.
Purification Method
Affinity purification
Gene ID
4706
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NDUFAB1 Rabbit pAb (CAB22019) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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