Anti-NDUFB2 Antibody (CAB3978)

Antibody Name:	Anti-NDUFB2 Antibody
Antibody SKU:	CAB3978
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 34-105 of human NDUFB2 (NP_004537.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460, HeLa, 293T, Mouse brain, Mouse heart, Mouse kidney, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 34-105 of human NDUFB2 (NP_004537.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AGGG VHIE PRYR QFPQ LTRS QVFQ SEFF SGLM WFWI LWRF WHDS EEVL GHFP YPDP SQWT DEEL GIPP DDED
Gene ID:	4708
Uniprot:	O95178
Cellular Location:	Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:	12kDa
Observed MW:	12kDa

Synonyms:

NDUFB2, AGGG, CI-AGGG

Background:

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays a important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Hydropathy analysis revealed that this subunit and 4 other subunits have an overall hydrophilic pattern, even though they are found within the hydrophobic protein (HP) fraction of complex I.

UniProt Protein Function:	NDUFB2: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB2 subunit family.
UniProt Protein Details:	Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 7q34 Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I Molecular Function:NADH dehydrogenase (ubiquinone) activity Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone
NCBI Summary:	The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays a important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Hydropathy analysis revealed that this subunit and 4 other subunits have an overall hydrophilic pattern, even though they are found within the hydrophobic protein (HP) fraction of complex I. [provided by RefSeq, Jul 2008]
UniProt Code:	O95178
NCBI GenInfo Identifier:	6093503
NCBI Gene ID:	4708
NCBI Accession:	O95178.1
UniProt Secondary Accession:	O95178,Q6FGI6,
UniProt Related Accession:	O95178
Molecular Weight:	105
NCBI Full Name:	NADH dehydrogenase
NCBI Synonym Full Names:	NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa
NCBI Official Symbol:	NDUFB2
NCBI Official Synonym Symbols:	AGGG; CI-AGGG
NCBI Protein Information:	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial; complex I-AGGG; complex I AGGG subunit; NADH-ubiquinone oxidoreductase AGGG subunit
UniProt Protein Name:	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial
UniProt Synonym Protein Names:	Complex I-AGGG; CI-AGGG; NADH-ubiquinone oxidoreductase AGGG subunit
UniProt Gene Name:	NDUFB2
UniProt Entry Name:	NDUB2_HUMAN

	Western blot analysis of extracts of various cell lines, using NDUFB2 antibody (CAB3978) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of L929 cells using NDUFB2 Polyclonal Antibody (CAB3978) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

NDUFB2 Antibody (PACO10787)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)