Western blot analysis of extracts of Rat heart, using NDUFS2 antibody (CAB21117) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The NDUFS2 Monoclonal Antibody (CAB21117) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced using hybridoma technology, exhibits high specificity and sensitivity for detecting NDUFS2 in various samples, making it an essential component in studies related to mitochondrial function and metabolism.The NDUFS2 protein is essential for the proper functioning of Complex I, the largest and most intricate enzyme complex in the mitochondrial electron transport chain. Mutations in NDUFS2 have been linked to various mitochondrial disorders and diseases, highlighting the importance of studying its function and regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NDUFS2 Monoclonal Antibody
SKU:
CAB21117
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC3018
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
4720
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat heart, using NDUFS2 Rabbit mAb (CAB21117) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Western blot analysis of various lysates, using NDUFS2 Rabbit mAb (CAB21117) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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