Anti-NEFH Antibody (CAB8442)

Antibody Name:	Anti-NEFH Antibody
Antibody SKU:	CAB8442
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 821-1020 of human NEFH (NP_066554.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 821-1020 of human NEFH (NP_066554.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KSPV KEEE KPQE VKVK EPPK KAEE EKAP ATPK TEEK KDSK KEEA PKKE APKP KVEE KKEP AVEK PKES KVEA KKEE AEDK KKVP TPEK EAPA KVEV KEDA KPKE KTEV AKKE PDDA KAKE PSKP AEKK EAAP EKKD TKEE KAKK PEEK PKTE AKAK EDDK TLSK EPSK PKAE KAEK SSST DQKD SKPP EKAT EDKA AKGK
Gene ID:	4744
Uniprot:	P12036
Cellular Location:
Calculated MW:	105kDa/112kDa
Observed MW:	240kDa

Synonyms:

CMT2CC, NFH, NEFH

Background:

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.

UniProt Protein Function:	NFH: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. Belongs to the intermediate filament family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: mitochondrion; axon; cytoplasm; neurofilament Molecular Function:protein binding, bridging; dynein binding; structural constituent of cytoskeleton; kinesin binding; microtubule binding; structural molecule activity; protein kinase binding Biological Process: axonogenesis; neurofilament bundle assembly; microtubule cytoskeleton organization and biogenesis; cell projection biogenesis; peripheral nervous system neuron axonogenesis Disease: Amyotrophic Lateral Sclerosis 1
NCBI Summary:	Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]
UniProt Code:	P12036
NCBI GenInfo Identifier:	226726294
NCBI Gene ID:	4744
NCBI Accession:	P12036.4
UniProt Secondary Accession:	P12036,Q96HF8, Q9UJS7, Q9UQ14, B4DYY4,
UniProt Related Accession:	P12036
Molecular Weight:
NCBI Full Name:	Neurofilament heavy polypeptide
NCBI Synonym Full Names:	neurofilament, heavy polypeptide
NCBI Official Symbol:	NEFH
NCBI Official Synonym Symbols:	NFH
NCBI Protein Information:	neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa
UniProt Protein Name:	Neurofilament heavy polypeptide
UniProt Synonym Protein Names:	200 kDa neurofilament protein; Neurofilament triplet H protein
Protein Family:	Neurofilament heavy polypeptide
UniProt Gene Name:	NEFH
UniProt Entry Name:	NFH_HUMAN

	Western blot analysis of extracts of mouse brain, using NEFH antibody (CAB8442) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded mouse kidney using NEFH antibody (CAB8442) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using NEFH antibody (CAB8442) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat cerebellum using NEFH antibody (CAB8442) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using NEFH Rabbit pAb (CAB8442) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-NEFH Antibody (CAB19084)	Human Neurofilament Heavy protein / NEFH ELISA Kit
NEFH Antibody (PACO04417)	Human Neurofilament heavy polypeptide (NEFH) ELISA Kit
NEFH Antibody (PACO10810)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)