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Anti-NELFE Antibody (CAB7006)

SKU:
CAB7006
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 4 Anti-NELFE Antibody CAB7006
  • Epigenetics and Nuclear Signaling Antibodies 4 Anti-NELFE Antibody CAB7006
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Description

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Antibody Name:Anti-NELFE Antibody
Antibody SKU:CAB7006
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NELFE (NP_002895.3).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human
Positive Samples:293T, HepG2, SW620
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NELFE (NP_002895.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLVI PPGL SEEE EALQ KKFN KLKK KKKA LLAL KKQS SSST TSQG GVKR SLSE QPVM DTAT ATEQ AKQL VKSG AISA IKAE TKNS GFKR SRTL EGKL KDPE KGPV PTFQ PFQR SISA DDDL QESS RRPQ RKSL YESF VSSS DRLR ELGP DGEE AEGP GAGD GPPR SFDW GYEE RSGA HSSA SPPR SRSR DRSH ERNR DRDR DRER DRDR DRDR DRER DRDR DRDR DRDR ERDR DRER DRDR DREG PFRR SDSF PERR APRK GNTL YVYG EDMT PTLL RGAF
Gene ID:7936
Uniprot:P18615
Cellular Location:Nucleus
Calculated MW:40kDa/43kDa
Observed MW:43kDa
Synonyms:NELFE, D6S45, NELF-E, RD, RDBP, RDP
Background:The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6.
UniProt Protein Function:RDBP: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Belongs to the RRM NELF-E family.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor; Mitochondrial

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; mitochondrion; nucleus

Molecular Function:protein binding; RNA binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression

NCBI Summary:The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008]
UniProt Code:P18615
NCBI GenInfo Identifier:1350554
NCBI Gene ID:7936
NCBI Accession:P18615.3
UniProt Related Accession:P18615
Molecular Weight:43kDa
NCBI Full Name:Negative elongation factor E
NCBI Synonym Full Names:negative elongation factor complex member E
NCBI Official Symbol:NELFE  
NCBI Official Synonym Symbols:RD; RDP; RDBP; D6S45; NELF-E  
NCBI Protein Information:negative elongation factor E
UniProt Protein Name:Negative elongation factor E
UniProt Synonym Protein Names:RNA-binding protein RD
UniProt Gene Name:NELFE  
UniProt Entry Name:NELFE_HUMAN
Anti-NELFE Antibody (CAB7006)Western blot analysis of extracts of various cell lines, using NELFE antibody (CAB7006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-NELFE Antibody (CAB7006)Immunofluorescence analysis of U2OS cells using NELFE antibody (CAB7006).

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