Anti-NKX2-5 Antibody (CAB5651)

Antibody Name:	Anti-NKX2-5 Antibody
Antibody SKU:	CAB5651
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human NKX2-5 (NP_004378.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Mouse brain, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human NKX2-5 (NP_004378.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MFPS PALT PTPF SVKD ILNL EQQQ RSLA AAGE LSAR LEAT LAPS SCML AAFK PEAY AGPE AAAP GLPE LRAE LGRA PSPA KCAS AFPA APAF YPRA YSDP DPAK DPRA EKKE LCAL QKAV ELEK TEAD NAER PRA
Gene ID:	1482
Uniprot:	P52952
Cellular Location:	Nucleus
Calculated MW:	11kDa/16kDa/34kDa
Observed MW:	37kDa

Synonyms:

NKX2-5, CHNG5, CSX, CSX1, HLHS2, NKX2.5, NKX2E, NKX4-1, VSD3

Background:

This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	NKX2-5: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Expressed only in the heart. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 5q34 Cellular Component: transcription factor complex; cytoplasm; nucleus Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; chromatin binding; transcription factor binding; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; heart morphogenesis; ventricular cardiac myofibril development; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell development; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; cardiac muscle cell differentiation; BMP signaling pathway; atrial cardiac muscle cell development; positive regulation of cell proliferation; cardiac muscle morphogensis; thyroid gland development; hemopoiesis; regulation of cardiac muscle cell proliferation; heart looping; cell differentiation; vasculogenesis; cardiac muscle contraction; spleen development; adult heart development; pharyngeal system development; sarcomere organization; positive regulation of cardioblast differentiation; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; negative regulation of transcription, DNA-dependent; positive regulation of heart contraction; negative regulation of apoptosis Disease: Ventricular Septal Defect 3; Atrial Septal Defect 7 With Or Without Atrioventricular Conduction Defects; Conotruncal Heart Malformations; Tetralogy Of Fallot; Hypothyroidism, Congenital, Nongoitrous, 5; Hypoplastic Left Heart Syndrome 2
NCBI Summary:	This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
UniProt Code:	P52952
NCBI GenInfo Identifier:	1708211
NCBI Gene ID:	1482
NCBI Accession:	P52952.1
UniProt Secondary Accession:	P52952,A8K3K0, B4DNB6, E9PBU6,
UniProt Related Accession:	P52952
Molecular Weight:	185
NCBI Full Name:	Homeobox protein Nkx-2.5
NCBI Synonym Full Names:	NK2 homeobox 5
NCBI Official Symbol:	NKX2-5
NCBI Official Synonym Symbols:	CSX; CSX1; VSD3; CHNG5; HLHS2; NKX2E; NKX2.5; NKX4-1
NCBI Protein Information:	homeobox protein Nkx-2.5; tinman paralog; homeobox protein CSX; cardiac-specific homeobox 1; homeobox protein NK-2 homolog E; NK2 transcription factor related, locus 5
UniProt Protein Name:	Homeobox protein Nkx-2.5
UniProt Synonym Protein Names:	Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E
Protein Family:	Homeobox protein
UniProt Gene Name:	NKX2-5
UniProt Entry Name:	NKX25_HUMAN

	Western blot analysis of extracts of various cell lines, using NKX2-5 antibody (CAB5651) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
	Immunofluorescence analysis of U2OS cells using NKX2-5 antibody (CAB5651) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-NKX2-5 Antibody (CAB12688)

NKX2-5 Antibody (PACO10865)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)