The NLRX1 Antibody (CAB4976) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has been rigorously tested for specificity and sensitivity in Western blot analysis, ensuring reliable and reproducible results. By targeting the NLRX1 protein, this antibody allows for the investigation of NLRX1 expression and function in various experimental settings.NLRX1 is a mitochondrial protein that plays a critical role in regulating inflammation and antiviral responses. Dysregulation of NLRX1 has been implicated in the pathogenesis of infectious diseases, autoimmune disorders, and metabolic disorders.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NLRX1 Antibody
SKU:
CAB4976
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NOD5, NOD9, NOD26, DLNB26, CLR11.3, NLRX1
Positive Sample:
Mouse heart
Cellular Localization:
Mitochondrion Outer Membrane.
Calculated MW:
108kDa
Observed MW:
108kDa
The protein encoded by this gene is a member of the NLR family and localizes to the outer mitochondrial membrane. The encoded protein is a regulator of mitochondrial antivirus responses. Three transcript variants encoding the same protein have been found for this gene.
Purification Method
Affinity purification
Gene ID
79671
RRID
AB_2765966
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse heart, using NLRX1 Rabbit pAb (CAB4976) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunoprecipitation analysis of 600 μg extracts of Mouse kidney using 3 μg NLRX1 antibody (CAB4976). Western blot was performed from the immunoprecipitate using NLRX1 (CAB4976) at a dilution of 1:1000.
