Anti-NONO Antibody (CAB5282)

Antibody Name:	Anti-NONO Antibody
Antibody SKU:	CAB5282
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NONO (NP_001138881.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 22Rv1, SW620, 293T, U2OS, Mouse spleen, Mouse brain, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NONO (NP_001138881.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MQSN KTFN LEKQ NHTP RKHH QHHH QQQH HQQQ QQQP PPPP IPAN GQQA SSQN EGLT IDLK NFRK PGEK TFTQ RSRL FVGN LPPD ITEE EMRK LFEK YGKA GEVF IHKD KGFG FIRL ETRT LAEI AKVE LDNM PLRG KQLR VRFA CHSA SLTV RNLP QYVS NELL EEAF SVFG QVER AVVI VDDR GRPS GKGI VEFS GKPA ARKA LDRC SEGS FLLT TFPR PVTV EPMD QLDD EEGL PEKL VIKN QQFH KERE QPPR FAQP GSFE YEYA MRWK ALIE MEKQ QQDQ VDRN IKEA REKL EMEM
Gene ID:	4841
Uniprot:	Q15233
Cellular Location:	Nucleus, Nucleus speckle, nucleolus
Calculated MW:	43kDa/54kDa
Observed MW:	58kDa

Synonyms:	NONO, MRXS34, NMT55, NRB54, P54, P54NRB, PPP1R114
Background:	This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.

UniProt Protein Function:	NONO: DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Together with PSPC1, required for the formation of nuclear paraspeckles. A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3.
UniProt Protein Details:	Protein type:RNA splicing; RNA-binding; Nucleolus; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: Xq13.1 Cellular Component: membrane; nuclear matrix; nucleoplasm; nucleus; paraspeckles Molecular Function:chromatin binding; identical protein binding; protein binding Biological Process: circadian rhythm; mRNA processing; negative regulation of transcription, DNA-dependent; nuclear mRNA splicing, via spliceosome; regulation of circadian rhythm; regulation of transcription, DNA-dependent; RNA splicing
NCBI Summary:	This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]
UniProt Code:	Q15233
NCBI GenInfo Identifier:	67469924
NCBI Gene ID:	4841
NCBI Accession:	Q15233.4
UniProt Secondary Accession:	Q15233,O00201, P30807, Q12786, Q9BQC5, B7Z4C2, D3DVV4 F5GYZ3,
UniProt Related Accession:	Q15233
Molecular Weight:	43,866 Da
NCBI Full Name:	Non-POU domain-containing octamer-binding protein
NCBI Synonym Full Names:	non-POU domain containing, octamer-binding
NCBI Official Symbol:	NONO
NCBI Official Synonym Symbols:	P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114
NCBI Protein Information:	non-POU domain-containing octamer-binding protein
UniProt Protein Name:	Non-POU domain-containing octamer-binding protein
UniProt Synonym Protein Names:	54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex, 52 kDa subunit; NMT55; p54(nrb); p54nrb
Protein Family:	Non-POU domain-containing octamer-binding protein
UniProt Gene Name:	NONO
UniProt Entry Name:	NONO_HUMAN

	Western blot analysis of extracts of various cell lines, using Nono antibody (CAB5282) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using NONO Rabbit pAb (CAB5282) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat liver using NONO Rabbit pAb (CAB5282) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using NONO Rabbit pAb (CAB5282) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human thyroid cancer using NONO Rabbit pAb (CAB5282) at dilution of 1:100 (40x lens).

Antibodies

Anti-MonoMethyl-NONO-R357/R365 Antibody (CAB8087)

Anti-NONO / p54nrb Antibody (CAB3800)

NONO Antibody (PACO10893)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)