The NOSIP Polyclonal Antibody (CAB21530) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding to NOSIP, this antibody enables the detection and analysis of NOSIP in various cell types, making it ideal for studies in molecular biology and cardiovascular research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NOSIP Polyclonal Antibody
SKU:
CAB21530
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CGI-25, NOSIP
Positive Sample:
293T
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
33kDa
Observed MW:
33kDa
The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein.
Purification Method
Affinity purification
Gene ID
51070
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and NOSIP knockout (KO) 293T cells using [KO Validated] NOSIP Rabbit pAb (CAB21530) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
