Anti-NOTCH2 Antibody (CAB0560)

Antibody Name:	Anti-NOTCH2 Antibody
Antibody SKU:	CAB0560
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2242-2471 of human NOTCH2 (NP_077719.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, LO2, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2242-2471 of human NOTCH2 (NP_077719.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SRLH PVPV PADW MNRM EVNE TQYN EMFG MVLA PAEG THPG IAPQ SRPP EGKH ITTP REPL PPIV TFQL IPKG SIAQ PAGA PQPQ STCP PAVA GPLP TMYQ IPEM ARLP SVAF PTAM MPQQ DGQV AQTI LPAY HPFP ASVG KYPT PPSQ HSYA SSNA AERT PSHS GHLQ GEHP YLTP SPES PDQW SSSS PHSA SDWS DVTT SPTP GGAG GGQR GPGT HMSE PPHN NMQV YA
Gene ID:	4853
Uniprot:	Q04721
Cellular Location:	Cell membrane, Cytoplasm, Nucleus, Single-pass type I membrane protein
Calculated MW:	265kDa
Observed MW:	120kDa

Synonyms:

NOTCH2, AGS2, HJCYS, hN2, notch 2

Background:

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	Notch 2: a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. Affects the implementation of differentiation, proliferation and apoptotic programs. Cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for the membrane bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus.
UniProt Protein Details:	Protein type:Receptor, misc.; Membrane protein, integral; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1p13-p11 Cellular Component: cell surface; endoplasmic reticulum membrane; extracellular region; Golgi membrane; integral to plasma membrane; membrane; nucleoplasm; nucleus; plasma membrane; receptor complex Molecular Function:protein binding Biological Process: apoptosis; bone remodeling; cell cycle arrest; cell fate determination; cell growth; hemopoiesis; marginal zone B cell differentiation; negative regulation of apoptosis; negative regulation of cell proliferation; Notch signaling pathway; organ morphogenesis; positive regulation of Ras protein signal transduction; regulation of transcription, DNA-dependent; stem cell maintenance; transcription initiation from RNA polymerase II promoter Disease: Alagille Syndrome 2; Hajdu-cheney Syndrome
NCBI Summary:	This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:	Q04721
NCBI GenInfo Identifier:	317008611
NCBI Gene ID:	4853
NCBI Accession:	NP_001186930.1
UniProt Secondary Accession:	Q04721,Q5T3X7, Q99734, Q9H240,
UniProt Related Accession:	Q04721
Molecular Weight:	Calculated MW: 265kDaObserved MW: 120kDa
NCBI Full Name:	neurogenic locus notch homolog protein 2 isoform 2
NCBI Synonym Full Names:	notch 2
NCBI Official Symbol:	NOTCH2
NCBI Official Synonym Symbols:	hN2; AGS2; HJCYS
NCBI Protein Information:	neurogenic locus notch homolog protein 2
UniProt Protein Name:	Neurogenic locus notch homolog protein 2
Protein Family:	Neurogenic locus notch homolog protein
UniProt Gene Name:	NOTCH2
UniProt Entry Name:	NOTC2_HUMAN

	Western blot analysis of extracts of various cell lines, using NOTCH2 antibody (CAB0560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human stomach using NOTCH2 antibody (CAB0560) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using NOTCH2 antibody (CAB0560) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using NOTCH2 antibody (CAB0560) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Cleaved-NOTCH2 (A1734) Antibody (PACO02934)	Human NOTCH2 ELISA Kit
Cleaved-NOTCH2 (V1697) Antibody (PACO04457)	Human Neurogenic locus notch homolog protein 2 (NOTCH2) ELISA Kit
Cleaved-NOTCH2 (D1733) Antibody (PACO06041)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)