The NOTCH3 Antibody (CAB0929) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.Notch3 plays a crucial role in various cellular processes, including differentiation, proliferation, and apoptosis. Dysregulation of Notch3 signaling has been implicated in a range of diseases, such as cancer, cardiovascular disorders, and neurological conditions. By targeting the Notch3 protein, researchers can gain valuable insights into the mechanisms driving these diseases and potentially identify novel therapeutic targets.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
NOTCH3 Antibody
SKU:
CAB0929
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IMF2, LMNS, CASIL, CADASIL, CADASIL1, NOTCH3
Positive Sample:
Rat skeletal muscle
Cellular Localization:
Cell Membrane, Nucleus, Single-Pass Type I Membrane Protein.
Calculated MW:
244kDa
Observed MW:
Refertofigures
This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
Purification Method
Affinity purification
Gene ID
4854
RRID
AB_2757465
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NOTCH3 Rabbit pAb (CAB0929) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 15s.
Immunofluorescence analysis of L929 cells using NOTCH3 Rabbit pAb (CAB0929) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
