Anti-NOTCH3 Antibody (CAB13522)

Antibody Name:	Anti-NOTCH3 Antibody
Antibody SKU:	CAB13522
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 2200 to the C-terminus of human NOTCH3 (NP_000426.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	OVCAR2, mouse ovary

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 2200 to the C-terminus of human NOTCH3 (NP_000426.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TPVS PQER PPPY LAVP GHGE EYPA AGAH SSPP KARF LRVP SEHP YLTP SPES PEHW ASPS PPSL SDWS ESTP SPAT ATGA MATT TGAL PAQP LPLS VPSS LAQA QTQL GPQP EVTP KRQV LA
Gene ID:	4854
Uniprot:	Q9UM47
Cellular Location:	Cell membrane, Nucleus, Single-pass type I membrane protein
Calculated MW:	243kDa
Observed MW:	280kDa

Synonyms:

NOTCH3, CADASIL, CADASIL1, CASIL, IMF2, LMNS, notch 3

Background:

This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).

UniProt Protein Function:	NOTCH3: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH3. Interacts with PSMA1. Interacts with HIF1AN. Ubiquitously expressed in fetal and adult tissues. Belongs to the NOTCH family.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 19p13.2-p13.1 Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cytoplasm; plasma membrane; extracellular region; integral to membrane; cytosol; receptor complex; actin cytoskeleton Molecular Function:protein binding; enzyme binding; calcium ion binding Biological Process: transcription initiation from RNA polymerase II promoter; negative regulation of neuron differentiation; Notch signaling pathway; regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; forebrain development; neuron fate commitment; Notch receptor processing; gene expression Disease: Cerebral Arteriopathy, Autosomal Dominant, With Subcortical Infarcts And Leukoencephalopathy; Lateral Meningocele Syndrome; Myofibromatosis, Infantile, 2
NCBI Summary:	This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UM47
NCBI GenInfo Identifier:	322510053
NCBI Gene ID:	4854
NCBI Accession:	Q9UM47.2
UniProt Secondary Accession:	Q9UM47,Q9UEB3, Q9UPL3, Q9Y6L8,
UniProt Related Accession:	Q9UM47
Molecular Weight:	2321
NCBI Full Name:	Neurogenic locus notch homolog protein 3
NCBI Synonym Full Names:	notch 3
NCBI Official Symbol:	NOTCH3
NCBI Official Synonym Symbols:	IMF2; CASIL; CADASIL
NCBI Protein Information:	neurogenic locus notch homolog protein 3; Notch homolog 3
UniProt Protein Name:	Neurogenic locus notch homolog protein 3
Protein Family:	Neurogenic locus notch homolog protein
UniProt Gene Name:	NOTCH3
UniProt Entry Name:	NOTC3_HUMAN

	Western blot analysis of extracts of various cell lines, using NOTCH3 antibody (CAB13522) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded mouse spleen using NOTCH3 antibody (CAB13522) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse pancreas using NOTCH3 antibody (CAB13522) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Human Notch3 Monoclonal Antibody (PE Conjugated) [MHN3-21](AGEL1859)	Human NOTCH3 ELISA Kit
Human Notch3 Monoclonal Antibody (APC Conjugated) [MHN3-21](AGEL1860)	Human Neurogenic locus notch homolog protein 3 (NOTCH3) ELISA Kit
Anti-NOTCH3 Antibody (CAB0929)
Anti-NOTCH3 Antibody (CAB3115)
NOTCH3 Antibody (PACO10900)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)