The NOX5 Antibody (CAB7136) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody is highly specific to human samples and has been validated for use in Western blot applications.NADPH oxidase 5 plays a crucial role in the immune system by generating ROS to help kill pathogens like bacteria and fungi. Dysregulation of this enzyme has been implicated in various inflammatory conditions and autoimmune diseases. The NADPH Oxidase 5 Polyclonal Antibody enables researchers to detect and quantify levels of NADPH oxidase 5 in different cell types, providing insights into its role in immune function and disease pathology.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NOX5 Antibody
SKU:
CAB7136
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Purification Method
Affinity purification
Gene ID
79400
RRID
AB_2767691
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from PC-3 cells, using NOX5 Rabbit pAb (CAB7136) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of A-549 cells using NOX5 Rabbit pAb (CAB7136) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
