The NPHS2 Antibody (CAB17337) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NPHS2 Antibody
SKU:
CAB17337
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PDCN, SRN1, NPHS2
Positive Sample:
NTERA-2, Rat spleen
Cellular Localization:
Cell-Cell Junction, Cytoplasmic Side Of Plasma Membrane, Endoplasmic Reticulum, Extracellular Exosome, Plasma Membrane, Slit Diaphragm.
Calculated MW:
42kDa
Observed MW:
42kDa
This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
7827
RRID
AB_2770633
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using NPHS2 Rabbit pAb (CAB17337) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
