Anti-NQO1 Antibody (CAB1518)

Antibody Name:	Anti-NQO1 Antibody
Antibody SKU:	CAB1518
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse,
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-50 of human NQO1 (NP_000894.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse,
Positive Samples:	A-549, HT-29

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-50 of human NQO1 (NP_000894.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVGR RALI VLAH SERT SFNY AMKE AAAA ALKK KGWE VVES DLYA MNFN PI
Gene ID:	1728
Uniprot:	P15559
Cellular Location:	Cytoplasm
Calculated MW:	26kDa/27kDa/30kDa
Observed MW:	32kDa

Synonyms:

DHQU, DIA4, DTD, NMOR1, NMORI, QR1, NQO1

Background:

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

UniProt Protein Function:	NQO1: a FAD-binding flavoprotein enzyme that that prevents the one electron reduction of quinones that results in the production of radical species. Involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Mutations have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues. Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development. A homozygous common missense variant (NQO1(*)2, rs1800566(T)), that disables NQO1 strongly predicts poor survival among two independent series of women with breast cancer. Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turn leads to myeloid hyperplasia of bone marrow. Altered expression is associated with Alzheimer's disease (AD). Inhibited by dicoumarol. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Oxidoreductase; EC 1.6.5.2 Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: cell soma; cytoplasm; cytosol Molecular Function:identical protein binding; protein binding; NAD(P)H dehydrogenase (quinone) activity; cytochrome-b5 reductase activity; superoxide dismutase activity Biological Process: response to ethanol; removal of superoxide radicals; response to toxin; xenobiotic metabolic process; positive regulation of neuron apoptosis; negative regulation of catalytic activity; regulation of amino acid metabolic process; nitric oxide biosynthetic process; response to nutrient; response to estradiol stimulus; aging; synaptic transmission, cholinergic
NCBI Summary:	This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	P15559
NCBI GenInfo Identifier:	118607
NCBI Gene ID:	1728
NCBI Accession:	P15559.1
UniProt Related Accession:	P15559
Molecular Weight:
NCBI Full Name:	NAD(P)H dehydrogenase
NCBI Synonym Full Names:	NAD(P)H quinone dehydrogenase 1
NCBI Official Symbol:	NQO1
NCBI Official Synonym Symbols:	DTD; QR1; DHQU; DIA4; NMOR1; NMORI
NCBI Protein Information:	NAD(P)H dehydrogenase [quinone] 1
UniProt Protein Name:	NAD(P)H dehydrogenase [quinone] 1
UniProt Synonym Protein Names:	Azoreductase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H:quinone oxidoreductase 1; Phylloquinone reductase; Quinone reductase 1; QR1
Protein Family:	NAD(P)H dehydrogenase
UniProt Gene Name:	NQO1
UniProt Entry Name:	NQO1_HUMAN

	Western blot analysis of extracts of various cell lines, using NQO1 antibody (CAB1518) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of L929 cells using NQO1 Rabbit pAb (CAB1518) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-NQO1 Antibody (CAB0047)	Human NQO1 ELISA Kit
Anti-NQO1 Antibody (CAB16830)	Human NAD(P)H dehydrogenase [quinone] 1 (NQO1) ELISA Kit
Anti-NQO1 Antibody (CAB19586)
NQO1 Antibody (PACO06698)
NQO1 Antibody (PACO10915)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)