Anti-NTRK1 Antibody (CAB2098)

Antibody Name:	Anti-NTRK1 Antibody
Antibody SKU:	CAB2098
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NTRK1 (NP_002520.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, K-562, NIH/3T3, Mouse liver, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NTRK1 (NP_002520.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLRG GRRG QLGW HSWA AGPG SLLA WLIL ASAG AAPC PDAC CPHG SSGL RCTR DGAL DSLH HLPG AENL TELY IENQ QHLQ HLEL RDLR GLGE LRNL TIVK SGLR FVAP DAFH FTPR LSRL NLSF NALE SLSW KTVQ GLSL QELV LSGN PLHC SCAL RWLQ RWEE EGLG GVPE QKLQ CHGQ GPLA HMPN ASCG VPTL KVQV PNAS VDVG DDVL LRCQ VEGR GLEQ AGWI LTEL EQSA TVMK SGGL PSLG LTLA NVTS DLNR KNVT CWAE NDVG RAEV SVQV
Gene ID:	4914
Uniprot:	P04629
Cellular Location:	Cell membrane, Early endosome membrane, Late endosome membrane, Single-pass type I membrane protein
Calculated MW:	77kDa/83kDa/86kDa/87kDa
Observed MW:	110kDa

Synonyms:

NTRK1, MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA

Background:

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.

UniProt Protein Function:	TrkA: a receptor tyrosine kinase of the Trk family. High affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor. Known substrates include Shc, PI-3K, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system. Two splice variant isoforms have been described.
UniProt Protein Details:	Protein type:Protein kinase, TK; Oncoprotein; EC 2.7.10.1; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; Trk family Chromosomal Location of Human Ortholog: 1q21-q22 Cellular Component: protein complex; cell surface; integral to plasma membrane; late endosome membrane; dendrite; early endosome; cell soma; early endosome membrane; axon; late endosome; plasma membrane; cytoplasmic vesicle; receptor complex; endosome Molecular Function:neurotrophin p75 receptor binding; neurotrophin binding; protein binding; nerve growth factor receptor activity; protein homodimerization activity; ephrin receptor binding; nerve growth factor binding; transmembrane receptor protein tyrosine kinase activity; ATP binding Biological Process: circadian rhythm; axon guidance; mechanoreceptor differentiation; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; positive regulation of synaptic transmission, glutamatergic; protein amino acid phosphorylation; olfactory nerve development; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; sympathetic nervous system development; response to radiation; learning and/or memory; small GTPase mediated signal transduction; response to axon injury; negative regulation of neuron apoptosis; response to electrical stimulus; detection of temperature stimulus involved in sensory perception of pain; response to hydrostatic pressure; aging; response to drug; response to nutrient levels; phosphoinositide-mediated signaling; adenylate cyclase activation; Sertoli cell development; detection of mechanical stimulus involved in sensory perception of pain; positive regulation of programmed cell death; positive regulation of angiogenesis; response to ethanol; phospholipase C activation; B cell differentiation; Ras protein signal transduction; response to activity; positive regulation of Ras protein signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway Disease: Thyroid Carcinoma, Familial Medullary; Insensitivity To Pain, Congenital, With Anhidrosis
NCBI Summary:	This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, cognitive disability and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]
UniProt Code:	P04629
NCBI GenInfo Identifier:	4585712
NCBI Gene ID:	4914
NCBI Accession:	NP_002520
UniProt Related Accession:	P04629
Molecular Weight:	87.511
NCBI Full Name:	high affinity nerve growth factor receptor isoform 2
NCBI Synonym Full Names:	neurotrophic receptor tyrosine kinase 1
NCBI Official Symbol:	NTRK1
NCBI Official Synonym Symbols:	MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
NCBI Protein Information:	high affinity nerve growth factor receptor
UniProt Protein Name:	High affinity nerve growth factor receptor
UniProt Synonym Protein Names:	Neurotrophic tyrosine kinase receptor type 1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor; Tyrosine kinase receptor A; Trk-A; gp140trk; p140-TrkA
Protein Family:	Trk system potassium uptake protein
UniProt Gene Name:	NTRK1
UniProt Entry Name:	NTRK1_HUMAN

	Western blot analysis of extracts of various cell lines, using NTRK1 antibody (CAB2098) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse stomach using NTRK1 Antibody (CAB2098) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat cerebellum using NTRK1 Antibody (CAB2098) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using TRKA antibody (CAB2098) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-NTRK1 Antibody (CAB15618)	Human High affinity nerve growth factor receptor (NTRK1) ELISA Kit
Anti-Phospho-NTRK1-S791 Antibody (CABP0272)	Trk A (Phospho-Tyr680+Tyr681) Colorimetric Cell-Based ELISA Kit
Anti-TrkA Antibody (CAB4147)	Trk A Colorimetric Cell-Based ELISA Kit
Anti-Phospho-TrkA-Y490 Antibody (CABP0492)	Trk A (Phospho-Tyr701) Colorimetric Cell-Based ELISA Kit
NTRK1 Antibody (PACO02939)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)