The NUAK1 Antibody (CAB17333) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and sensitive for detecting NUAK1 in human samples, making it suitable for a variety of research applications such as Western blotting and immunofluorescence.NUAK1, also known as AMPK-related kinase 5, is a key player in various cellular processes including energy metabolism, cell cycle regulation, and stress response.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
NUAK1 Antibody
SKU:
CAB17333
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ARK5, NUAK1
Positive Sample:
rat heart
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
74kDa
Observed MW:
74kDa
Enables p53 binding activity and protein serine/threonine kinase activity. Involved in several processes, including protein phosphorylation; regulation of cellular senescence; and regulation of myosin-light-chain-phosphatase activity. Located in cytoplasm; microtubule cytoskeleton; and nuclear lumen.
Purification Method
Affinity purification
Gene ID
9891
RRID
AB_2770680
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from rat heart, using NUAK1 Rabbit pAb (CAB17333) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
