Anti-NUP210 Antibody (CAB16676)

Antibody Name:	Anti-NUP210 Antibody
Antibody SKU:	CAB16676
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 680-880 of human NUP210 (NP_079199.2).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 680-880 of human NUP210 (NP_079199.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QNVT AEDT DSIG LALF APHS SRNY QQHW ILVT CQAL GEQV IALS VGNK PSLT NPFP AVEP AVVK FVCA PPSR LTLA PVYT SPQL DMSC PLLQ QNKQ VVPV SSHR NPRL DLAA YDQE GRRF DNFS SLSI QWES TRPV LASI EPEL PMQL VSQD DESG QKKL HGLQ AILV HEAS GTTA ITAT ATGY QESH LSSA RTKQ PHDP L
Gene ID:	23225
Uniprot:	Q8TEM1
Cellular Location:
Calculated MW:	205kDa
Observed MW:	250kDa

Synonyms:

NUP210, GP210, POM210

Background:

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a membrane-spanning glycoprotein that is a major component of the nuclear pore complex. Multiple pseudogenes related to this gene are located on chromosome 3.

UniProt Protein Function:	NUP210: Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity. Belongs to the NUP210 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nucleoporin; Membrane protein, integral Chromosomal Location of Human Ortholog: 3p25.1 Cellular Component: membrane; nuclear envelope; nuclear pore Biological Process: mitotic nuclear envelope disassembly; mRNA export from nucleus; protein sumoylation; RNA-mediated gene silencing; tRNA export from nucleus; viral reproduction; viral transcription
NCBI Summary:	The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a membrane-spanning glycoprotein that is a major component of the nuclear pore complex. Multiple pseudogenes related to this gene are located on chromosome 3. [provided by RefSeq, Jul 2013]
UniProt Code:	Q8TEM1
NCBI GenInfo Identifier:	116242720
NCBI Gene ID:	23225
NCBI Accession:	Q8TEM1.3
UniProt Secondary Accession:	Q8TEM1,O94980, Q6NXG6, Q8NBJ1, Q9H6C8, Q9UFP3, A6NN56
UniProt Related Accession:	Q8TEM1
Molecular Weight:	105,810 Da
NCBI Full Name:	Nuclear pore membrane glycoprotein 210
NCBI Synonym Full Names:	nucleoporin 210
NCBI Official Symbol:	NUP210
NCBI Official Synonym Symbols:	GP210; POM210
NCBI Protein Information:	nuclear pore membrane glycoprotein 210
UniProt Protein Name:	Nuclear pore membrane glycoprotein 210
UniProt Synonym Protein Names:	Nuclear envelope pore membrane protein POM 210; POM210; Nucleoporin Nup210; Pore membrane protein of 210 kDa
Protein Family:	Nuclear pore membrane glycoprotein
UniProt Gene Name:	NUP210
UniProt Entry Name:	PO210_HUMAN

	Western blot analysis of extracts of various cell lines, using NUP210 antibody (CAB16676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of L929 cells using NUP210 antibody (CAB16676) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using NUP210 antibody (CAB16676) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-NUP210 Antibody (CAB19895)[KO Validated]

NUP210 Antibody (PACO10970)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)