Anti-OAS3 Antibody (CAB9481)

Antibody Name:	Anti-OAS3 Antibody
Antibody SKU:	CAB9481
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 320-620 of human OAS3 (NP_006178.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, DU145, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 320-620 of human OAS3 (NP_006178.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AASC YDHP CFLR GMGD PVQS WKGP GLPR AGCS GLGH PIQL DPNQ KTPE NSKS LNAV YPRA GSKP PSCP APGP TGAA SIVP SVPG MALD LSQI PTKE LDRF IQDH LKPS PQFQ EQVK KAID IILR CLHE NCVH KASR VSKG GSFG RGTD LRDG CDVE LIIF LNCF TDYK DQGP RRAE ILDE MRAQ LESW WQDQ VPSL SLQF PEQN VPEA LQFQ LVST ALKS WTDV SLLP AFDA VGQL SSGT KPNP QVYS RLLT SGCQ EGEH KACF AELR RNFM NIRP VKLK NLIL LVKH WYRQ VAAQ NKGK G
Gene ID:	4940
Uniprot:	Q9Y6K5
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	121kDa
Observed MW:	121kDa

Synonyms:	OAS3, p100, p100OAS
Background:	This gene encodes an enzyme included in the 2', 5' oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2', 5' oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance.

UniProt Protein Function:	OAS3: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes preferentially dimers of 2'-5'- oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. Displays antiviral activity against Chikungunya virus (CHIKV), Dengue virus, Sindbis virus (SINV) and Semliki forest virus (SFV). Belongs to the 2-5A synthase family.
UniProt Protein Details:	Protein type:EC 2.7.7.84; Transferase Chromosomal Location of Human Ortholog: 12q24.2 Cellular Component: cytoplasm; cytosol; extracellular space; intracellular membrane-bound organelle; nucleoplasm; plasma membrane Molecular Function:2'-5'-oligoadenylate synthetase activity; ATP binding; double-stranded RNA binding; protein binding Biological Process: defense response to virus; negative regulation of viral genome replication; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; response to virus
NCBI Summary:	This gene encodes an enzyme included in the 2', 5' oligoadenylate synthase family. This enzyme is induced by interferons and catalyzes the 2', 5' oligomers of adenosine in order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition of cellular protein synthesis and viral infection resistance. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y6K5
NCBI GenInfo Identifier:	317373408
NCBI Gene ID:	4940
NCBI Accession:	Q9Y6K5.3
UniProt Secondary Accession:	Q9Y6K5,Q2HJ14, Q9H3P5,
UniProt Related Accession:	Q9Y6K5
Molecular Weight:	121,170 Da
NCBI Full Name:	2'-5'-oligoadenylate synthase 3
NCBI Synonym Full Names:	2'-5'-oligoadenylate synthetase 3
NCBI Official Symbol:	OAS3
NCBI Official Synonym Symbols:	p100; p100OAS
NCBI Protein Information:	2'-5'-oligoadenylate synthase 3
UniProt Protein Name:	2'-5'-oligoadenylate synthase 3
UniProt Synonym Protein Names:	p100 OAS; p100OAS
Protein Family:	2'-5'-oligoadenylate synthase
UniProt Gene Name:	OAS3
UniProt Entry Name:	OAS3_HUMAN

	Western blot analysis of extracts of various cell lines, using OAS3 antibody (CAB9481) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of L929 cells using OAS3 antibody (CAB9481) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using OAS3 antibody (CAB9481) at dilution of 1:100. Blue: DAPI for nuclear staining.

ELISA Kits

Human OAS3 / 2'-5'-oligoadenylate synthase 3 ELISA Kit

Human 2'-5'-oligoadenylate synthase 3 (OAS3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)