Anti-Occludin Antibody (CAB2601)

Antibody Name:	Anti-Occludin Antibody
Antibody SKU:	CAB2601
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 263-522 of human Occludin (NP_002529.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Mouse brain, Mouse kidney, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 263-522 of human Occludin (NP_002529.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FAVK TRRK MDRY DKSN ILWD KEHI YDEQ PPNV EEWV KNVS AGTQ DVPS PPSD YVER VDSP MAYS SNGK VNDK RFYP ESSY KSTP VPEV VQEL PLTS PVDD FRQP RYSS GGNF ETPS KRAP AKGR AGRS KRTE QDHY ETDY TTGG ESCD ELEE DWIR EYPP ITSD QQRQ LYKR NFDT GLQE YKSL QSEL DEIN KELS RLDK ELDD YREE SEEY MAAA DEYN RLKQ VKGS ADYK SKKN HCKQ LKSK LSHI KKMV GDYD RQKT
Gene ID:	100506658
Uniprot:	Q16625
Cellular Location:	Cell junction, Membrane, Multi-pass membrane protein, tight junction
Calculated MW:	8kDa/23kDa/31kDa/52kDa/54kDa/59kDa
Observed MW:	59kDa

Synonyms:

OCLN, BLCPMG, PPP1R115, PTORCH1, occludin, Occludin

Background:

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.

UniProt Protein Function:	occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 5q13.1 Cellular Component: tight junction; endocytic vesicle; apicolateral plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; cytoplasmic vesicle; intercellular junction; cytosol; cell junction Molecular Function:thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; protein complex assembly; S-adenosylhomocysteine metabolic process; cell structure disassembly during apoptosis Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria
NCBI Summary:	This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
UniProt Code:	Q16625
NCBI GenInfo Identifier:	3914196
NCBI Gene ID:	100506658
NCBI Accession:	Q16625.1
UniProt Secondary Accession:	Q16625,Q5U1V4, Q8N6K1, B5BU70, D2DU64, D2DU65, D2IGC0 D2IGC1, E2CYV9,
UniProt Related Accession:	Q16625
Molecular Weight:	522
NCBI Full Name:	Occludin
NCBI Synonym Full Names:	occludin
NCBI Official Symbol:	OCLN
NCBI Official Synonym Symbols:	BLCPMG; PPP1R115
NCBI Protein Information:	occludin; tight junction protein occludin; phosphatase 1, regulatory subunit 115
UniProt Protein Name:	Occludin
Protein Family:	Occludin
UniProt Gene Name:	OCLN
UniProt Entry Name:	OCLN_HUMAN

	Western blot analysis of extracts of various cell lines, using Occludin antibody (CAB2601) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat ovary using Occludin Rabbit pAb (CAB2601) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using Occludin Rabbit pAb (CAB2601) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-Occludin Antibody (CAB12621)	Human OCLN / Occludin ELISA Kit
OCLN Antibody (PACO10990)	Human OCLN (Occludin) CLIA Kit (HUES00631)
	Human OCLN (Occludin) ELISA Kit (HUES02170)
	Human Occludin (OCLN) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)