The BGLAP Antibody (CAB6205) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, specifically targets osteocalcin and is highly reactive with human samples, making it ideal for use in Western blot applications.Osteocalcin is a marker for bone turnover and is involved in the regulation of bone mineralization, making it a crucial factor in bone health and disease. By utilizing the Osteocalcin Polyclonal Antibody, researchers can accurately detect and analyze osteocalcin levels in various cell types, providing valuable insights into bone metabolism and potential therapeutic targets for conditions such as osteoporosis and bone fractures.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
BGLAP Antibody
SKU:
CAB6205
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
OC, BGP, OCN, Osteocalcin (BGLAP)
Positive Sample:
U-87MG, Rat lung
Cellular Localization:
Secreted.
Calculated MW:
11kDa
Observed MW:
12kDa
This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product.
Purification Method
Affinity purification
Gene ID
632
RRID
AB_2766815
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from U-87MG cells, using Osteocalcin (BGLAP) Rabbit pAb (CAB6205) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from Rat lung, using Osteocalcin (BGLAP) Rabbit pAb (CAB6205) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of NIH/3T3 cells using Osteocalcin (BGLAP) Rabbit pAb (CAB6205) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Osteocalcin (BGLAP) Rabbit pAb (CAB6205) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
