The OT (Oxytocin) ELISA Kit is specifically designed for the precise quantification of oxytocin levels in human serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and accuracy, guaranteeing consistent and dependable results, which makes it perfect for various research purposes.Oxytocin is a critical hormone involved in social bonding, maternal behavior, and stress regulation. It plays a vital role in various physiological processes, including childbirth, lactation, and emotional responses. Oxytocin has also been implicated in mental health disorders, autism, and bonding behaviors, making it a valuable biomarker for investigating these conditions and exploring potential treatments.To learn more about the OT (Oxytocin) ELISA Kit, please visit: www.assaygenie.com/ot-oxytocin-elisa-kit-unes00019/
Product Name:
OT (Oxytocin) ELISA Kit
SKU:
UNES00019
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
