Anti-P2RY6 Antibody (CAB2485)

Antibody Name:	Anti-P2RY6 Antibody
Antibody SKU:	CAB2485
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant protein of human P2RY6

Application:	WB
Recommended Dilution:	WB 1:500 - 1:1000
Reactivity:	Human, Mouse
Positive Samples:	MCF7, Mouse lung, Mouse brain

Immunogen:	Recombinant protein of human P2RY6
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	5031
Uniprot:	Q15077
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	36kDa
Observed MW:	42kDa

Synonyms:

P2RY6, P2Y6

Background:

The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, which is a G-protein coupled receptor, is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. It is proposed that this receptor mediates inflammatory responses. Alternative splicing results in multiple transcript variants that encode different protein isoforms.

UniProt Protein Function:	P2Y6: Receptor for extracellular UDP > UTP > ATP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR Chromosomal Location of Human Ortholog: 11q13.5 Cellular Component: integral to plasma membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane Molecular Function:G-protein coupled receptor activity; UDP-activated nucleotide receptor activity; purinergic nucleotide receptor activity, G-protein coupled Biological Process: transepithelial chloride transport; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of smooth muscle cell migration
NCBI Summary:	The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, which is a G-protein coupled receptor, is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. It is proposed that this receptor mediates inflammatory responses. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Mar 2013]
UniProt Code:	Q15077
NCBI GenInfo Identifier:	2495018
NCBI Gene ID:	5031
NCBI Accession:	Q15077.1
UniProt Related Accession:	Q15077
Molecular Weight:	36kDa
NCBI Full Name:	P2Y purinoceptor 6
NCBI Synonym Full Names:	pyrimidinergic receptor P2Y6
NCBI Official Symbol:	P2RY6
NCBI Official Synonym Symbols:	P2Y6
NCBI Protein Information:	P2Y purinoceptor 6
UniProt Protein Name:	P2Y purinoceptor 6
UniProt Gene Name:	P2RY6
UniProt Entry Name:	P2RY6_HUMAN

Western blot analysis of extracts of various cell lines, using P2RY6 antibody (CAB2485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

Anti-P2Y6 Antibody (CAB3708)

P2RY6 Antibody (PACO11041)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)