Western blot analysis of HACAT, using PADI1/PAD1 Rabbit mAb (CAB23951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The PADI1/PAD1 Monoclonal Antibody (CAB23951) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, is highly specific to human PADI1 and is validated for use in various applications including Western blot and immunohistochemistry.PADI1, also known as peptidylarginine deiminase 1, is involved in the citrullination of proteins, a process that has been implicated in autoimmune diseases such as rheumatoid arthritis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PADI1/PAD1 Monoclonal Antibody
SKU:
CAB23951
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC61569
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
HaCaT, 293T transfected with PADI1/PAD1, Mouse ovary
Cellular Localization:
Cytoplasm.
Calculated MW:
74kDa
Observed MW:
75kDa
This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type I enzyme is involved in the late stages of epidermal differentiation, where it deiminates filaggrin and keratin K1, which maintains hydration of the stratum corneum, and hence the cutaneous barrier function. This enzyme may also play a role in hair follicle formation. This gene exists in a cluster with four other paralogous genes.
Purification Method
Affinity purification
Gene ID
29943
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HaCaT cells using PADI1/PAD1 Rabbit mAb (CAB23951) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from Mouse ovary using PADI1/PAD1 Rabbit mAb (CAB23951) at 1:1400 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020) Exposure time: 60 s.
