The PALD1 Antibody (CAB12893) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity for human samples and has been validated for use in Western blotting applications. By binding to PALD1, this antibody enables the detection and analysis of PALD1 protein levels in various cell types, making it a useful tool for investigations in cell biology and cancer research.PALD1, also known as paladin, is a key player in cell adhesion and migration processes, making it an attractive target for research into cancer metastasis and tissue repair.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PALD1 Antibody
SKU:
CAB12893
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PALD, KIAA1274, PALD1
Positive Sample:
Mouse kidney, Mouse lung, Mouse heart, Mouse liver, Rat kidney
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
97kDa
Observed MW:
97kDa
Predicted to enable protein tyrosine phosphatase activity. Predicted to be involved in peptidyl-tyrosine dephosphorylation. Located in cytosol.
Purification Method
Affinity purification
Gene ID
27143
RRID
AB_2759737
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PALD1 Rabbit pAb (CAB12893) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of L929 cells using PALD1 Rabbit pAb (CAB12893) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
