The SPG7 Antibody (CAB10249) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, derived from rabbits, exhibits high specificity for human paraplegin and is validated for use in Western blot applications.Paraplegin is a mitochondrial protein involved in ATP-dependent proteolysis, playing a crucial role in mitochondrial quality control and cellular homeostasis. Dysfunctional paraplegin has been linked to neurodegenerative disorders such as HSP, making it a target of interest for therapeutic interventions aimed at preserving mitochondrial function.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SPG7 Antibody
SKU:
CAB10249
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified.
Purification Method
Affinity purification
Gene ID
6687
RRID
AB_2757775
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SPG7 Rabbit pAb (CAB10249) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 10s.
Immunofluorescence analysis of HepG2 cells using SPG7 Rabbit pAb (CAB10249) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
