Anti-PARN Antibody (CAB4002)

Antibody Name:	Anti-PARN Antibody
Antibody SKU:	CAB4002
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PARN (NP_002573.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	MCF7, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PARN (NP_002573.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEII RSNF KSNL HKVY QAIE EADF FAID GEFS GISD GPSV SALT NGFD TPEE RYQK LKKH SMDF LLFQ FGLC TFKY DYTD SKYI TKSF NFYV FPKP FNRS SPDV KFVC QSSS IDFL ASQG FDFN KVFR NGIP YLNQ EEER QLRE QYDE KRSQ ANGA GALS YVSP NTSK CPVT IPED QKKF IDQV VEKI EDLL QSEE NKNL DLEP CTGF QRKL IYQT LSWK YPKG IHVE TLET EKKE RYIV ISKV DEEE RKRR EQQK HAKE QEEL NDAV GFSR VIHA IANS
Gene ID:	5073
Uniprot:	O95453
Cellular Location:	Cytoplasm, Nucleus, nucleolus
Calculated MW:	52kDa/66kDa/67kDa/73kDa
Observed MW:	73kDa

Synonyms:

PARN, DAN, DKCB6, PFBMFT4

Background:

The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PARN: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization. Homodimer. Interacts with KHSRP and CELF1/CUGBP1. Found in a mRNA decay complex with RENT1, RENT2 and RENT3B. Ubiquitous. Belongs to the CAF1 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ribonuclease; RNA-binding; EC 3.1.13.4; Nucleolus Chromosomal Location of Human Ortholog: 16p13 Cellular Component: cytoplasm; nucleolus; cytosol; nucleus Molecular Function:protein binding; poly(A)-specific ribonuclease activity; nuclease activity; mRNA 3'-UTR binding; metal ion binding; nucleotide binding; protein kinase binding Biological Process: poly(A) tail shortening; RNA modification; mRNA catabolic process, nonsense-mediated decay; female gamete generation; gene expression; mRNA catabolic process, deadenylation-dependent decay Disease: Pulmonary Fibrosis And/or Bone Marrow Failure, Telomere-related, 4; Dyskeratosis Congenita, Autosomal Recessive, 6
NCBI Summary:	The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:	O95453
NCBI GenInfo Identifier:	60390262
NCBI Gene ID:	5073
NCBI Accession:	O95453.1
UniProt Related Accession:	O95453
Molecular Weight:	~ 73kDa
NCBI Full Name:	Poly(A)-specific ribonuclease PARN
NCBI Synonym Full Names:	poly(A)-specific ribonuclease
NCBI Official Symbol:	PARN
NCBI Official Synonym Symbols:	DAN; DKCB6; PFBMFT4
NCBI Protein Information:	poly(A)-specific ribonuclease PARN
UniProt Protein Name:	Poly(A)-specific ribonuclease PARN
UniProt Synonym Protein Names:	Deadenylating nuclease; Deadenylation nuclease; Polyadenylate-specific ribonuclease
Protein Family:	Poly(A)-specific ribonuclease
UniProt Gene Name:	PARN
UniProt Entry Name:	PARN_HUMAN

	Western blot analysis of extracts of various cell lines, using PARN antibody (CAB4002) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 150s.
	Immunohistochemistry of paraffin-embedded human esophageal cancer using PARN antibody (CAB4002) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using PARN antibody (CAB4002). Blue: DAPI for nuclear staining.

Antibodies

Anti-PARN Antibody (CAB6941)

PARN Antibody (PACO11072)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)