The Pyruvate carboxylase (PC) Monoclonal Antibody (CAB8980) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and sensitive for detecting PCB molecules in various samples, making it ideal for use in a range of research applications.With its high affinity and specificity for PCB, this monoclonal antibody allows for accurate and reliable detection and quantification of PCB levels in environmental samples, biological tissues, and other relevant materials. Its versatility in applications such as ELISA, immunohistochemistry, and flow cytometry makes it a valuable asset for researchers investigating the impact of PCB exposure on human health and the environment.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Pyruvate carboxylase (PC) Monoclonal Antibody
SKU:
CAB8980
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC1371
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PCB, PC, Pyruvate carboxylase (PC)
Positive Sample:
Mouse liver, Mouse kidney, Rat liver
Cellular Localization:
Mitochondrion Matrix.
Calculated MW:
130kDa
Observed MW:
130kDa
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.
Purification Method
Affinity purification
Gene ID
5091
RRID
AB_2863636
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Pyruvate carboxylase (PC) Rabbit mAb (CAB8980) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
