Anti-PDC Antibody (CAB14654)

Antibody Name:	Anti-PDC Antibody
Antibody SKU:	CAB14654
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human PDC (NP_002588.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse eye, Rat eye

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human PDC (NP_002588.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEEA KSQS LEED FEGQ ATHT GPKG VIND WRKF KLES QDSD SIPP SKKE ILRQ MSSP QSRN GKDS KERV SRKM SIQE YELI HKEK EDEN CLRK YRRQ CMQD MHQK LSFG PRYG FVYE LETG KQFL ETIE KELK ITTI VVHI YEDG IKGC DALN SSLT CLAA EYPI VKFC KIKA SNTG AGDR FSLD VLPT LLIY KGGE LISN FISV AEQF AEEF FAGD VESF LNEY GLLP EREV HVLE HTKI EEED VE
Gene ID:	5132
Uniprot:	P20941
Cellular Location:	Cell projection, Cytoplasm, Nucleus, Nucleus, Photoreceptor inner segment, cilium, cytosol, photoreceptor outer segment
Calculated MW:	22kDa/28kDa/32kDa
Observed MW:	28kDa

Synonyms:

PDC, MEKA, PHD, PhLOP, PhLP, phosducin

Background:

This gene encodes a phosphoprotein, which is located in the outer and inner segments of the rod cells in the retina. This protein may participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism. It modulates the phototransduction cascade by interacting with the beta and gamma subunits of the retinal G-protein transducin. This gene is a potential candidate gene for retinitis pigmentosa and Usher syndrome type II. Alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	phosducin: May participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism. Inhibits the transcriptional activation activity of the cone-rod homeobox CRX. Belongs to the phosducin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:G protein regulator, misc. Chromosomal Location of Human Ortholog: 1q25.2 Cellular Component: cytoplasm Molecular Function:phospholipase inhibitor activity; queuine tRNA-ribosyltransferase activity Biological Process: G-protein coupled receptor protein signaling pathway; phototransduction; queuosine biosynthetic process
NCBI Summary:	This gene encodes a phosphoprotein, which is located in the outer and inner segments of the rod cells in the retina. This protein may participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism. It modulates the phototransduction cascade by interacting with the beta and gamma subunits of the retinal G-protein transducin. This gene is a potential candidate gene for retinitis pigmentosa and Usher syndrome type II. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	P20941
NCBI GenInfo Identifier:	130134
NCBI Gene ID:	5132
NCBI Accession:	P20941.1
UniProt Secondary Accession:	P20941,Q14816, Q9UP22, Q9UP23,
UniProt Related Accession:	P20941
Molecular Weight:	32,118 Da
NCBI Full Name:	Phosducin
NCBI Synonym Full Names:	phosducin
NCBI Official Symbol:	PDC
NCBI Official Synonym Symbols:	PHD; MEKA; PhLP; PhLOP
NCBI Protein Information:	phosducin
UniProt Protein Name:	Phosducin
UniProt Synonym Protein Names:	33 kDa phototransducing protein; Protein MEKA
Protein Family:	Phosducin
UniProt Gene Name:	PDC
UniProt Entry Name:	PHOS_HUMAN

	Western blot analysis of extracts of various cell lines, using PDC Rabbit pAb (CAB14654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat retina using PDC antibody (CAB14654) at dilution of 1:200 (40x lens).

Antibodies

Anti-Phosducin Antibody (CAB8447)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)