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Anti-PDGFD Antibody (CAB8263)

SKU:
CAB8263
Product Type:
Antibody
Applications:
WB
IF
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 12 Anti-PDGFD Antibody CAB8263
  • Cell Biology Antibodies 12 Anti-PDGFD Antibody CAB8263
€139 - €419
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Description

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Antibody Name:PDGFD Rabbit Polyclonal Antibody
Antibody SKU:CAB8263
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 185-364 of human PDGFD (NP_149126.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:A-549, THP-1, Mouse kidney, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 185-364 of human PDGFD (NP_149126.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SGVS YNSP SVTD PTLI ADAL DKKI AEFD TVED LLKY FNPE SWQE DLEN MYLD TPRY RGRS YHDR KSKV DLDR LNDD AKRY SCTP RNYS VNIR EELK LANV VFFP RCLL VQRC GGNC GCGT VNWR SCTC NSGK TVKK YHEV LQFE PGHI KRRG RAKT MALV DIQL DHHE RCDC ICSS RPPR
Gene ID:80310
Uniprot:Q9GZP0
Cellular Location:Secreted
Calculated MW:42kDa
Observed MW:43kDa
Synonyms:PDGFD, IEGF, MSTP036, SCDGF-B, SCDGFB
Background:The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene.
UniProt Protein Function:PDGFD: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; Oncoprotein

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: Golgi membrane; endoplasmic reticulum lumen; extracellular region

Molecular Function:growth factor activity; platelet-derived growth factor receptor binding

Biological Process: multicellular organismal development; positive regulation of cell division; platelet-derived growth factor receptor signaling pathway; regulation of peptidyl-tyrosine phosphorylation

NCBI Summary:The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9GZP0
NCBI GenInfo Identifier:13376808
NCBI Gene ID:80310
NCBI Accession:NP_079484.1
UniProt Secondary Accession:Q9GZP0,Q9BWV5, A8K9T6,
UniProt Related Accession:Q9GZP0
Molecular Weight:
NCBI Full Name:platelet-derived growth factor D isoform 1
NCBI Synonym Full Names:platelet derived growth factor D
NCBI Official Symbol:PDGFD  
NCBI Official Synonym Symbols:IEGF; SCDGFB; MSTP036; SCDGF-B  
NCBI Protein Information:platelet-derived growth factor D
UniProt Protein Name:Platelet-derived growth factor D
UniProt Synonym Protein Names:Iris-expressed growth factor; Spinal cord-derived growth factor B; SCDGF-B
Protein Family:Platelet-derived growth factor
UniProt Gene Name:PDGFD  
UniProt Entry Name:PDGFD_HUMAN
Anti-PDGFD Polyclonal Antibody (CAB8263)Western blot analysis of extracts of various cell lines, using PDGFD antibody (CAB8263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-PDGFD Polyclonal Antibody (CAB8263)Immunofluorescence analysis of U2OS cells using PDGFD antibody (CAB8263) at dilution of 1:100. Blue: DAPI for nuclear staining.

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