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Anti-PDGFRA Antibody (CAB2103)

SKU:
CAB2103
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 8 Anti-PDGFRA Antibody CAB2103
  • Cell Biology Antibodies 8 Anti-PDGFRA Antibody CAB2103
€139 - €419
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Description

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Antibody Name:Anti-PDGFRA Antibody
Antibody SKU:CAB2103
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human PDGFRA (NP_006197.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human PDGFRA (NP_006197.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGTS HPAF LVLG CLLT GLSL ILCQ LSLP SILP NENE KVVQ LNSS FSLR CFGE SEVS WQYP MSEE ESSD VEIR NEEN NSGL FVTV LEVS SASA AHTG LYTC YYNH TQTE ENEL EGRH IYIY VPDP DVAF VPLG MTDY LVIV EDDD SAII PCRT TDPE TPVT LHNS EGVV PASY DSRQ GFNG TFTV GPYI CEAT VKGK KFQT IPFN VYAL KA
Gene ID:5156
Uniprot:P16234
Cellular Location:Cell membrane, Single-pass type I membrane protein
Calculated MW:24kDa/82kDa/122kDa
Observed MW:190kDa
Synonyms:PDGFRA, CD140A, PDGFR-2, PDGFR2, GAS9, RHEPDGFRA
Background:This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
UniProt Protein Function:PDGFRA: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation. Particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. Chromosomal rearrangments activate PDGFRalpha by fusion to BCR, causing atypical chronic myelogenous leukemia (CML), and to FIP1L1, causing idiopathic hypereosinophilic syndrome. Activating point mutations cause a minority of gastrointestinal stromal tumors (GIST). Promoter polymorphisms linked to neural tube defects including spina bifida, verified by mouse mutant model. Inhibitors: Gleevec, Sutent. OMIM: Two alternatively-spliced isoforms have been described.
UniProt Protein Details:

Protein type:Protein kinase, tyrosine (receptor); Oncoprotein; Kinase, protein; Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; TK group; PDGFR family

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: microvillus; membrane; integral to plasma membrane; cytoplasm; plasma membrane; nucleus; intrinsic to plasma membrane; external side of plasma membrane

Molecular Function:vascular endothelial growth factor receptor activity; protein binding; protein homodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; platelet-derived growth factor alpha-receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: estrogen metabolic process; extracellular matrix organization and biogenesis; peptidyl-tyrosine phosphorylation; regulation of chemotaxis; wound healing; nerve growth factor receptor signaling pathway; viral reproduction; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; cardiac myofibril assembly; palate development; elevation of cytosolic calcium ion concentration; positive regulation of fibroblast proliferation; Leydig cell differentiation; embryonic digestive tract morphogenesis; luteinization; positive regulation of cell proliferation; male genitalia development; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; in utero embryonic development; adrenal gland development; positive regulation of phosphoinositide 3-kinase activity; embryonic cranial skeleton morphogenesis; odontogenesis of dentine-containing teeth; embryonic skeletal morphogenesis; positive regulation of phosphoinositide 3-kinase cascade; cell activation; innate immune response; hemopoietic progenitor cell differentiation; positive regulation of DNA replication; lung development; positive regulation of cell migration

Disease: Gastrointestinal Stromal Tumor; Hypereosinophilic Syndrome, Idiopathic

NCBI Summary:This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. [provided by RefSeq, Mar 2012]
UniProt Code:P16234
NCBI GenInfo Identifier:129892
NCBI Gene ID:5156
NCBI Accession:P16234.1
UniProt Secondary Accession:P16234,Q6P4H5, Q96KZ7, Q9UD28, B2RE69, E9PBH0,
UniProt Related Accession:P16234
Molecular Weight:1089
NCBI Full Name:Platelet-derived growth factor receptor alpha
NCBI Synonym Full Names:platelet-derived growth factor receptor, alpha polypeptide
NCBI Official Symbol:PDGFRA  
NCBI Official Synonym Symbols:CD140A; PDGFR2; PDGFR-2; RHEPDGFRA  
NCBI Protein Information:platelet-derived growth factor receptor alpha; PDGF-R-alpha; CD140a antigen; PDGFRA/BCR fusion; CD140 antigen-like family member A; platelet-derived growth factor receptor 2; alpha-type platelet-derived growth factor receptor; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha fusion protein
UniProt Protein Name:Platelet-derived growth factor receptor alpha
UniProt Synonym Protein Names:Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; PDGFR-2; CD_antigen: CD140a
Protein Family:Platelet-derived growth factor receptor
UniProt Gene Name:PDGFRA  
UniProt Entry Name:PGFRA_HUMAN
Anti-PDGFRA Antibody (CAB2103)Western blot analysis of extracts of various cell lines, using PDGFRA antibody (CAB2103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PDGFRA Antibody (CAB2103)Immunofluorescence analysis of U-2 OS cells using PDGFRA Rabbit pAb (CAB2103) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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