Anti-PDK3 Antibody (CAB8028)

Antibody Name:	PDK3 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8028
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 137-406 of human PDK3 (NP_005382.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Mouse brain, Mouse spleen, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 137-406 of human PDK3 (NP_005382.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FGFD PFIS TNIQ YFLD RFYT NRIS FRML INQH TLLF GGDT NPVH PKHI GSID PTCN VADV VKDA YETA KMLC EQYY LVAP ELEV EEFN AKAP DKPI QVVY VPSH LFHM LFEL FKNS MRAT VELY EDRK EGYP AVKT LVTL GKED LSIK ISDL GGGV PLRK IDRL FNYM YSTA PRPS LEPT RAAP LAGF GYGL PISR LYAR YFQG DLKL YSME GVGT DAVI YLKA LSSE SFER LPVF NKSA WRHY KTTP EADD WSNP SSEP RDAS KYKA KQ
Gene ID:	5165
Uniprot:	Q15120
Cellular Location:	Mitochondrion matrix
Calculated MW:	46kDa/48kDa
Observed MW:	47kDa

Synonyms:

PDK3, CMTX6, GS1-358P8.4

Background:

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PDHK3: an atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK3 is present in testes, kidney, and brain. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.
UniProt Protein Details:	Protein type:EC 2.7.11.2; Protein kinase, atypical; Kinase, protein; Mitochondrial; ATYPICAL group; PDHK family Chromosomal Location of Human Ortholog: Xp22.11 Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:ATP binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity Biological Process: peptidyl-serine phosphorylation; regulation of acetyl-CoA biosynthetic process from pyruvate
NCBI Summary:	The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
UniProt Code:	Q15120
NCBI GenInfo Identifier:	3183119
NCBI Gene ID:	5165
NCBI Accession:	Q15120.1
UniProt Secondary Accession:	Q15120,B4DXG6,
UniProt Related Accession:	Q15120
Molecular Weight:	48,043 Da
NCBI Full Name:	[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial
NCBI Synonym Full Names:	pyruvate dehydrogenase kinase 3
NCBI Official Symbol:	PDK3
NCBI Official Synonym Symbols:	CMTX6; GS1-358P8.4
NCBI Protein Information:	pyruvate dehydrogenase kinase, isozyme 3
UniProt Protein Name:	[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial
UniProt Synonym Protein Names:	Pyruvate dehydrogenase kinase isoform 3
UniProt Gene Name:	PDK3
UniProt Entry Name:	PDK3_HUMAN

	Western blot analysis of extracts of various cell lines, using PDK3 antibody (CAB8028) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded mouse lung using PDK3 antibody (CAB8028) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using PDK3 antibody (CAB8028) at dilution of 1:100 (40x lens).

Antibodies

Anti-PDK3 Antibody (CAB12480)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)