The PE-GenieFluor 594 Anti-Mouse CD162 Antibody (4RA10) is a valuable tool for researchers studying CD162, a cell surface antigen involved in immune cell adhesion and migration. This antibody, produced in mice, offers high specificity and sensitivity in detecting CD162 in mouse samples, making it suitable for flow cytometry and immunofluorescence applications.
By targeting the CD162 protein, researchers can explore its role in immune cell interactions and its potential as a therapeutic target in inflammatory and autoimmune diseases. Overall, the PE-GenieFluor 594 Anti-Mouse CD162 Antibody (4RA10) is a crucial asset for investigations into immune cell function and pathology.
P-selectin glycoprotein ligand 1;Selplg;PSGL-1;Selectin P ligand;CD162;
Host Species:
Rat
Reactivity:
Mouse
Application:
FCM
Isotype:
Rat IgG1, κ
Isotype Control:
PE/Genie Fluor 594 Rat IgG1, κ Isotype Control[HRPN]
Form:
Liquid
Conjugation:
PE/Genie Fluor594
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
Q62170
Gene ID:
20345
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.
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