The CD31/PECAM1 Antibody (CAB0378) is a high-quality antibody developed for reliable detection and analysis of target proteins. This cell surface protein is involved in various cellular processes, including cell adhesion, migration, and signaling. The antibody, raised in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications.PECAM1 is known to play a key role in vascular biology, particularly in mediating interactions between endothelial cells and platelets. Its expression on both platelets and endothelial cells makes it a crucial player in processes like thrombosis, inflammation, and angiogenesis.
This antibody is validated for use in WB, IHC-P, IF-P applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CD31/PECAM1 Antibody
SKU:
CAB0378
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cell Junction, Cell Junction, Cell Membrane, Lipid-Anchor, Single-Pass Type I Membrane Protein.
Calculated MW:
83kDa
Observed MW:
140kDa
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
Purification Method
Affinity purification
Gene ID
5175
RRID
AB_2757159
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from THP-1 cells, using CD31/PECAM1 Rabbit pAb (CAB0378) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from Mouse heart, using CD31/PECAM1 Rabbit pAb (CAB0378) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from U-937 cells, using CD31/PECAM1 Rabbit pAb (CAB0378) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD31/PECAM1 Rabbit pAb (CAB0378) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of Human placenta tissue using CD31/PECAM1 Rabbit pAb (CAB0378) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.
