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Phospho-ACLY-S455 Rabbit Polyclonal Antibody (CABP0779)

SKU:
CABP0779
Product Type:
Antibody
Applications:
WB
IHC
IP
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
  • Metabolism Antibodies 3 Anti-Phospho-ACLY-S455 pAb Antibody CABP0779
  • Metabolism Antibodies 3 Anti-Phospho-ACLY-S455 pAb Antibody CABP0779
  • Metabolism Antibodies 3 Anti-Phospho-ACLY-S455 pAb Antibody CABP0779
  • Metabolism Antibodies 3 Anti-Phospho-ACLY-S455 pAb Antibody CABP0779
  • Metabolism Antibodies 3 Anti-Phospho-ACLY-S455 pAb Antibody CABP0779
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-ACLY-S455 Rabbit Polyclonal Antibody
SKU:CABP0779
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S455 of human ACLY (NP_001087.2).
Sequence:RTAS FS
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:WB,1:100 - 1:500 IHC-P,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:ACL; ATPCL; CLATP; Phospho-ACLY-S455
Positive Sample:C6,NIH/3T3,HeLa
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:121kDa
Observed MW:125kDa

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

Purification Method:Affinity purification
Gene ID:47
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from C6 cells using Phospho-ACLY-S455 Rabbit pAb (CABP0779) at 1:400 dilution. C6 cells were treated by Insulin (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

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