Anti-Phospho-ACLY-S455 pAb Antibody (CABP0779)
- SKU:
- CABP0779
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- IP
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-Phospho-ACLY-S455 Antibody |
| Antibody SKU: | CABP0779 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S455 of human ACLY (NP_001087.2). |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NIH/3T3 |
| Immunogen: | A synthetic phosphorylated peptide around S455 of human ACLY (NP_001087.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TASF S |
| Gene ID: | 47 |
| Uniprot: | P53396 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 91kDa/119kDa/120kDa |
| Observed MW: | 121kDa |
 | Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ACLY-S455 antibody (CABP0779) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat pancreas using Phospho-ACLY-S455 antibody (CABP0779) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using Phospho-ACLY-S455 antibody (CABP0779) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse pancreas using Phospho-ACLY-S455 antibody (CABP0779) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells, using 3 ug Anti-Phospho-ACLY-S455 pAb (CABP0779). Western blot was performed from the immunoprecipitate using Anti-Phospho-ACLY-S455 pAb (CABP0779) at a dilition of 1:1000. NIH/3T3 cells were treated by Insulin (100 nM) at 37'C for 10 minutes after serum-starvation overnight. |
| Synonyms: | ACLY, ACL, ATPCL, CLATP |
| Background: | ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440, 000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. |
| UniProt Protein Function: | ACLY: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Homotetramer. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.3.3.8; Transferase; Lyase; Carbohydrate Metabolism - citrate (TCA) cycle Chromosomal Location of Human Ortholog: 17q21.2 Cellular Component: cytoplasm; cytosol; membrane; mitochondrion; nucleoplasm; plasma membrane Molecular Function:ATP binding; ATP citrate synthase activity; cofactor binding; metal ion binding; protein binding Biological Process: acetyl-CoA biosynthetic process; cellular lipid metabolic process; cholesterol biosynthetic process; citrate metabolic process; energy reserve metabolic process; fatty acid biosynthetic process; lipid biosynthetic process; oxaloacetate metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process |
| NCBI Summary: | ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014] |
| UniProt Code: | P53396 |
| NCBI GenInfo Identifier: | 116241237 |
| NCBI Gene ID: | 47 |
| NCBI Accession: | P53396.3 |
| UniProt Secondary Accession: | P53396,Q13037, Q9BRL0, B4DIM0, B4E3P0, |
| UniProt Related Accession: | P53396 |
| Molecular Weight: | 91,099 Da |
| NCBI Full Name: | ATP-citrate synthase |
| NCBI Synonym Full Names: | ATP citrate lyase |
| NCBI Official Symbol: | ACLYÂ Â |
| NCBI Official Synonym Symbols: | ACL; ATPCL; CLATPÂ Â |
| NCBI Protein Information: | ATP-citrate synthase |
| UniProt Protein Name: | ATP-citrate synthase |
| UniProt Synonym Protein Names: | ATP-citrate (pro-S-)-lyase; ACL; Citrate cleavage enzyme |
| Protein Family: | ATP-citrate synthase |
| UniProt Gene Name: | ACLYÂ Â |
| UniProt Entry Name: | ACLY_HUMAN |
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