Description
Phospho-B-Raf-S445 Antibody (CABP0012)
The Phospho-B-Raf-S445 Antibody (CABP0012) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity in detecting phosphorylation of BRAF at Serine 445 in human samples, making it suitable for Western blot analysis.BRAF is a critical protein kinase involved in cell growth, proliferation, and differentiation, with aberrant activation contributing to tumorigenesis. Phosphorylation at Serine 445 has been implicated in regulating BRAF activity and downstream signaling pathways, making it a crucial post-translational modification to study in cancer biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
| Product Name: | Phospho-B-Raf-S445 Antibody |
| SKU: | CABP0012 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | RDSS D | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | NS7, B-raf, BRAF1, RAFB1, B-RAF1, BRAF-1, Phospho-B-Raf-S445 |
| Positive Sample: | Raji treated with PMA |
| Cellular Localization: | Cell Membrane, Cytoplasm, Nucleus. |
| Calculated MW: | 84kDa |
| Observed MW: | 100kDa |
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
| Purification Method | Affinity purification |
| Gene ID | 673 |
| RRID | AB_2770946 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from Raji cells, using Phospho-B-Raf-S445 Rabbit pAb (CAB0038). Raji cells were treated with PMA/TPA (200nM) for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s. |
